The magnitude of inhibition by these metabolites is affected by the levels of Mg 2+. The oligomeric form of the enzyme is more resistant to citrate inhibition than the monomeric form. In the presence of citrate or ammonium sulphate, the kinetics of the oligomeric form of PFK with F6P ...
Influence of 2,3-diphosphoglycerate on phosphofructokinase of human erythrocytes?Phosphofructokinase catalyzes the conversion offructose-6-phosphate to fructose-1,6-diphosphate.Control of glycolysis is usually explained in terms ofthe properties of three enzymes: hexokinase, phosphofructokinaseand pyruvate ...
Escherichia coli contains a major phosphofructokinase isoenzyme, phosphofructokinase 1, which is allosteric, and a minor isoenzyme, phosphofructokinase 2. The pfkBl mutation is known to increase the amount of phosphofructokinase 2 and allow growth on sugars of mutants lacking phosphofructokinase 1; it ...
Phosphofructokinase-2 was purified to homogeneity from chicken livers by homogeniza-tion, polyethylene glycol fractionation and column chromatography on DEAE-Sephadex A-50 and Blue-Sepharose 4B. Some properties of the enzyme were as follows: (i) The saturation curve of the enzyme for fructose 6-...
We aimed to explore the role of T helper cell 1 (Th1)/Th2 imbalance mediated by T cell glycolytic rate-limiting enzymes hexokinase 2 (HK2), phosphofructokinase-1 (PEK1) and pyruvate kinase M2 (PKM2) in oral lichen planus (OLP).#A total of 120 OLP patients treated in our hospital and...
2,3-diphosphoglyceraterat hemoglobinWe have studied the erythrocyte enzyme phosphofructokinase (PFK) from two strains of Long-Evans rats with genetically determined differences in erythrocyte 2,3-diphosphoglycerate (DPG) levels. The DPG difference is due to two alleles at one locus. With one ...
Citrate produces increases in the K0.5 for Fru-6-P and in the Hill coefficient as well as a decrease in the Vmax of the reaction without affecting the kinetic parameters for ATP as substrate. ATP potentiates synergistically the effects of citrate as an inhibitor of the enzyme. Fru-2,6-P2...
Phosphofructokinase-1 from Saccharomyces cerevisiae is composed of two types of subunits, and β. Subunit-specific monoclonal antibodies were raised to elucidate structural and functional properties of both subunits. One monoclonal antibody, -F3, binds to an epitope either at the C-terminal or at ...
These studies relate to a working hypothesis that storage is facilitated in resting muscle by inhibiting glycolysis via inhibition of , AK, and -1 by ; when muscle is active, these isozymes combine with muscle and are released and protected from inhibition by and glycolysis proceeds. Focus in ...
10(2): pp. 382-3.Yang, Z., Goronzy, J. J. & Weyand, C. M. The glycolytic enzyme PFKFB3/phosphofructokinase regulates autophagy. Autophagy 10, 382-383, doi: 10.4161/auto.27345 (2014).The glycolytic enzyme PFKFB3/phosphofructokinase regulates autophagy. Yang Z,Goronzy JJ,Weyand CM. ...