Phosphodiester Bond in DNA & RNA | Linkage, Formation & Function from Chapter 6 / Lesson 5 80K Understand what a phosphodiester bond is, learn about phosphodiester bond formation, explore the role of phosphodiester bonds in DNA and...
The invention provides methods and compositions for improving the fidelity of a sequencing-by-synthesis reaction by using a nucleotide derivative that forms a hydrogen bond with a complementary nucleotide on a template, but fails to form a phosphodiester bond with the 3′ hydroxyl group of a ...
In 1995, Wengel and collaborators described the synthesis of DNA oligonucleotides with 1–3 acyclic unlocked thymidine nucleotides (7, Chart 2, B=Thy, a dinucleotide is shown), that have an OH group at the 2′ position, yet lack a covalent bond between the 2′ and 3′ carbon atoms [6]...
While for the final compounds with a terminal amino group, a fork-shaped band appeared at 3300–3500 cm−1 and this band corresponded to the –NH2 bond stretching of the terminal amino group. Mass spectra showed correct molecular ion peaks. The isotopic pattern was used to confirm the ...