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PathScan® Phospho-c-Kit (Tyr719) Sandwich ELISA Antibody Pair 规格 1 Kit 货号 GOYK96591 抗体的制备过程: 1. 免疫原的制备 普通的大分子蛋白,通过分子克隆构建载体并在大肠杆菌中进行诱导表达获得重组蛋白,纯化鉴定后可直接作为免疫原。 小分子蛋白或化合物等分子量小,需要偶联载体对该分子进行改造才能使其...
c-myc 0.38 0.40 0.49 6.7 0.39 10.0 a ND, not determined. To assess the effect of serine Ser-202 phosphorylation on HuR/mRNA binding, a HuR/mRNA co-immunoprecipitation assay was employed on HuR S202A and HuR S202D induced clones. The mRNAs co-precipitated with HuR mutants (FLAG antibody-...
Samples were boiled for 5 min and 10 μg of protein were separated by SDS-PAGE, transferred to PVDF membrane using eBlot™ L1 Protein Transfer System (Genscript), blocked with 5% BSA (Sigma) prepared in 0.1% TBS-Tween20 and probed with the relevant primary antibody overnight at 4 °C...
anti-GFP (sc-9996, Santa Cruz), anti-influenza A virus NP (A01506, GenScript), anti-phosphothreonine (9381, Cell Signaling Technology, CST). Mouse monoclonal anti-influenza A virus M1 antibody was kindly provided (17) Evolutionary Analysis and Functional Identification of Ancient Brass...
Negative control for IHC was done by overnight incubation with BSA instead of primary antibody followed by incubation with respective secondary antibody. For color development, diaminobenzidine (sc-24982, SantaCruz Biotechnology, Santa Cruz, CA, USA) was used, with hematoxylin as a counterstain. ...
molecular sciencesAugust 15, 2023Urushihara Y, Hashimoto T, Fujishima Y, Hosoi Y.antibody (#NB100-104,Novus Biologicals, LLC, Englewood, CO, USA); phospho-ATM (Ser1981) antibody (#5883,CST); DNA-PKcs antibody (#sc-9051,Santa Cruz Biotechnology, Inc., Dallas, TX, USA); phospho-DN...
2c). Interestingly, the antibody did not react with human ATG4A in the presence of ULK1 (Supplementary Fig. 1). However, this does not rule out that other ATG4 members are not phosphorylated by ULK1 as the phospho-antibody may not crossreact with the slightly different motif. In fact, ...
anti-phospho-tyrosine antibody, PY99 (Santa Cruz Biotechnology, sc7020) diluted 1:100 in 1% (w/v) BSA, 25 mm Tris pH 7.5, 0.5 m NaCl, 0.1% Tween 20, 25 mm NaF, and 1 mm Na3VO4 for 16 h at 4 °C followed by secondary antibody and development with ECL Prime (GE Healthcare)...
Cells lysates were subjected to Western blot analysis with specific antibody against various PLCβ isoforms. β-actin was used as loading control. *Simultaneous knockdown the Gβγ-responsive PLCβ2 and PLCβ3 isoforms resulted in significant suppression of SDF-1α-induced chemotaxis. (B) HeLa ...