Phenol/chloroform extraction is a common technique used to separate and purify DNA, RNA, and protein from a biological specimen, such as avirus preparation. It involves the differential partitioning of DNA/protein and RNA into organic and aqueous phases, respectively. First, a monophasic solution ...
6) phenol and chloroform method 酚仿抽提法 1. The DNA extraction from the hairs of the Panthera Tigris Altaica,cooking extraction method in water was studied,and the comparison with the traditional phenol and chloroform method was done. 研究东北虎毛发DNA的水煮抽提法,并将传统的酚仿抽提法和...
1) phenol-chloroform extraction 酚氯仿法1. falciparum was extracted by phenol-chloroform extraction and amplified by loop-mediated isothermal amplification(LAMP) using 4 primers recognizing 6 distinct regions on the CSP gene of P. 方法酚氯仿法提取恶性疟原虫基因组DNA,设计四条扩增恶性疟原虫环子孢子...
Phenol-ChloroformIsoamylAlcohol(PCI)DNAExtraction ModifiedfromprotocolsbyBarkeretal(1998)AttheBench. Overview:Thisprotocoldescribesthemostcommonlyusedmethodofpurifyingand concentratingDNAfromsamples.TheDNAsolutionisfirstextractedwitha phenol/chloroform/isoamylalcoholmixturetoremoveproteincontaminants,then ...
A Phenol-Chloroform Protocol for Extracting DNA from Ancient Samples. In: Shapiro B, Hofreiter M, Walker JM, eds. Ancient DNA.Vol 840. Methods in Molecular Biology. Humana Press; 2012:13-19. Available at: http://www.springerlink.com/content/hv56230569205m05/abstract/. Accessed May 12, ...
If you''re in a hurry, you can shorten the protocol to two phenol extractions and one chloroform extraction. The number of repetitions also depends on what kind of sample you have and what you want to do with it. If you have whole cell extracts you want to use more phenol steps as ...
The fourth method, based on PEG precipitation followed by phenol/chloroform extraction with the addition of the detergent cetyltrimethylammonium bromide (CTAB)... AD Hale,J Green,DWG Brown - 《Journal of Virological Methods》 被引量: 168发表: 1996年 The preparation and physical chemical properties...
A modified phenol- chloroform extraction method for isolating circulating cell free DNA of tumor patients. J Nucleic Acids Investig. 2013;4:1.Hufnagl C., Stocher M., Moik M., Geisberger R., Greil R. A modified Phenol-chloroform extraction method for isolating circulating cell free DNA of ...
Modified phenol-chloroform method to extract the dna characteristics of high purity and high recovery rate may be certain application value in the trace samples dna extraction. 翻译结果2复制译文编辑译文朗读译文返回顶部 Before and after treatment with methylprednisolone data no significant statistical differ...
The Direct-zol™-96 MagBead RNA kit provides a high-throughput (96-well), magnetic bead-based isolation of high-quality RNA directly from samples in TRI Reagent®and similar. The extraction method inactivates viruses and other infectious agents. Total RNA including......