The genomic DNA extraction method,cooking extraction method in water,was studied and the comparison with the traditional phenol and chloroform method was done. 研究哺乳动物基因组DNA的水煮抽提法,并将传统的酚仿抽提法和水煮法进行比较,水煮法即通过对细胞的煮沸和冷却,使细胞破裂、蛋白变性,从而获得用于PCR...
Objective To establish an effective phenol-chloroform method coupled with paramagnetic particle method for human DNA extraction from maggot crop contents in STR genotyping. 目的建立运用酚-氯仿法结合磁珠法从蝇蛆嗉囊内提取人类DNA的方法,从而提高STR分型检验的灵敏度。 更多例句>> 4...
The present invention provides an improved phenol - chloroform extraction of fungal DNA, said method comprising the fungal cell lysis and protein digestion separation step DNA extraction; method of the invention is the use of SDS, proteinase K and other chemical substances effective combination, ...
Phenol-ChloroformIsoamylAlcohol(PCI)DNAExtraction ModifiedfromprotocolsbyBarkeretal(1998)AttheBench. Overview:Thisprotocoldescribesthemostcommonlyusedmethodofpurifyingand concentratingDNAfromsamples.TheDNAsolutionisfirstextractedwitha phenol/chloroform/isoamylalcoholmixturetoremoveproteincontaminants,then precipitatedwith100...
Below is atotal RNA isolationprotocol using the phenol/chloroform extraction method. For optimal results, special attention should be paid to the removal of RNase contamination. Disposable reagents like microfuge tubes should be sterile and RNase-free. Work surfaces and non-disposable tools and instrum...
Phenol/chloroform extraction of mostly genomic DNA from different tissue samples. Left: mouse tail sample; right: parallel extraction from mouse liver sample. Equilibrated phenol can typically be purchased from commercial sources. Alternatively, you can equilibrate it yourself. Be advised that this is ...
Modified phenol-chloroform method to extract the dna characteristics of high purity and high recovery rate may be certain application value in the trace samples dna extraction. 翻译结果2复制译文编辑译文朗读译文返回顶部 Before and after treatment with methylprednisolone data no significant statistical differ...
4) saturated phenol-chloroform extraction method 饱和苯酚-氯仿抽提法5) Tris-NH4Cl method Tris-NH4Cl法 1. ObjectiveTo study the relationship between Tris-NH4Cl method and isinglass method in assaying lymphocyte micronucleus rate in peripheral blood. [目的]研究Tris-NH4Cl法与明胶法检测人外周血淋巴...
The Direct-zol™-96 MagBead RNA kit provides a high-throughput (96-well), magnetic bead-based isolation of high-quality RNA directly from samples in TRI Reagent®and similar. The extraction method inactivates viruses and other infectious agents. Total RNA including......
In molecular biology, it is used in the extraction of nucleic acid from tissues by using the liquid/liquid phenol-chloroform extraction technique. It is an active component of paint strippers which are used for the removal of epoxy and polyurethane. It is also used in the preparation of ...