which stands for Synthesis phase; and G2, which stands for Gap phase 2. Interphase is the first of two phases of the eukaryotic cell cycle. The second phase is mitosis, or M phase, which is when cell division occurs. Sometimes cells do not leave G1because they are not the type of ce...
Oligos were purified by phenol/chloroform extraction, concentrated by ethanol precipitation, resuspended in 0.1 M sodium borate, and labeled with Cy3B (GE Healthcare) or Alexa647 NHS-ester (Life Technologies). After another ethanol precipitation, labeled oligos were resuspended in water and labeling ...
The upregulated DEGs in the proliferative vs. secretory endocervix ranked by fold change are listed the Supplementary Table2. The complete data can be found in the NCBI’s Gene Expression Omnibus database (Accession Number:GSE122248)29.FOSL1, a regulator of cell proliferation and differentiation,...
The M phase in cell division is the mitotic phase in the cell cycle. In this phase, the cell undergoes a major reorganization of almost all of the cell components. The chromosomes condense, the nuclear envelope surrounding the cell as a cell wall breaks down, and the cytoskeleton changes to...
To distinguish between the two cell types in fluorescence microscopy, the dental pulp cells were transduced to express eGFP, and the epithelial keratinocytes were labeled with CellTracker Red. After four weeks of 3D culture, the two cell types were tightly grown together with a proportion of red...
of proteins with labeled DNA at all stages of mitosis. We used CAA inDrosophilaembryos at nuclear divisions 14–16 to detect the association of proteins with DNA during mitosis. At this developmental stage, the cell cycles are very short (S phase is 50 min, G2 is from 30 to 150 ...