To evaluate antigen-specific cell division of iPSC-derived T-cell, we labeled WT1-TCR-transduced iPSC-T cells (iT cells), and CD19 iCART cells with CellTrace Violet (Termo Fisher Scientific). We then stimulated a total of 1 × 105iT cells or iCART cells with a total of 1 ×...
In summary, our analyses ofhcASDgenes (Fig.4) and emerging work on newly predicted ASD risk genes highlight two major human neural development epochs. First, these genes are highly active during early fetal stages of cell proliferation, differentiation, migration and early organization. Second, the...
The proportion of blastocysts comprising inner cells derived from the differentiative divisions during 32- to 64-cell transition was assessed based on the presence of beads-labeled inner (ICM) cells. We found that ICM cells derived from outer blastomeres, which underwent differentiative division durin...
Different stages of liver regeneration. Hepatocytes are stimulated by other cells and start to proliferate; the proliferating hepatocyte clusters in turn stimulate the proliferation of other cells; the damaged liver returns to its normal structure Full size image The liver lobule is the basic histologic...
Cells are color-labeled by cell states (left) and inferred pseudotime (right). Note the bifurcation of progenitor into pre-LC1 and pre-LC2, then into adjacent LC1 and LC2 subsets. (E) Wave-like dynamics of the five co-expressed gene sets across the entire differentiation trajectory. Top, ...
These data will enable people to clearly understand the molecular map of all stages of the disease and promote the development of accurate medical strategies in clinical treatment. First of all, the accurate analysis of the expression, localization information and functional changes of tens of ...
Injury to contractile organs such as the heart, vasculature, urinary bladder and gut can stimulate a pathological response that results in loss of normal contractility. PDGF and TGFβ are among the most well studied initiators of the injury response and
Even though in OncM mice the T cells appear to go through the same developmental stages as regular thymocytes, they are less functional, which may be the result of positive selection by other hematopoietic cells instead of thymic epithelium [25]. This, however, remains to be elucidated for T...
a Human B-ALL cells acquired from two patients at distinct disease stages (chemotherapy naïve and chemotherapy relapsed) were genetically labeled with DNA barcodes and transplanted into irradiated immunocompromised mice. Leukemia progression was monitored through peripheral blood analysis. b Human chimeris...
stages. diffHic finds TAD boundaries are generally weakened just prior to the first division (Fig.2b) while TADbit finds that TADs become larger (Fig.2c) and less numerous (Fig.2d, e) as a consequence at the same time point. This is likely reflective of the loss of TAD structure known...