T-cell division To evaluate antigen-specific cell division of iPSC-derived T-cell, we labeled WT1-TCR-transduced iPSC-T cells (iT cells), and CD19 iCART cells with CellTrace Violet (Termo Fisher Scientific). We then stimulated a total of 1 × 105 iT cells or iCART cells with a ...
cells was highly varied among donors, with most being found in fetal sample F7. However, there was no significant difference between the number of epicardial cells in fetal or adult donors, suggesting that the proportion of epicardial cells is not markedly different between stages (unpaired Student...
(a) Schematic diagram of injecting PRP-loaded DECHS into a nude mouse; (b) in vivo fluorescence imaging following subcutaneous injection of DIO-labeled cells in the control group, DECHS group, and PRP-loaded DECHS group. (c) Quantitative diagram of the DIO signal intensity expressed as ...
Genes with adjusted P < 0.05 for each cell-type pair were labeled as significantly differentially expressed. Venny diagram Venny (https://bioinfogp.cnb.csic.es/tools/venny/index.html) was applied to analysis genes which were specially expressed in cambium and primary xylem cells of Jin668...
These models are broadly applied in applications like object tracking, edge detection, shape modeling, segmentation, finding the mobility of the active objects, and handle the cell division by providing a primary segmentation for the first frame (Sacan et al., 2008b). Tracking by model ...
Cells are color-labeled by cell states (left) and inferred pseudotime (right). Note the bifurcation of progenitor into pre-LC1 and pre-LC2, then into adjacent LC1 and LC2 subsets. (E) Wave-like dynamics of the five co-expressed gene sets across the entire differentiation trajectory. Top, ...
Eight gene sets were derived from the microarray analysis encompassing gene sets with differential expression during the tachyzoite and bradyzoite stages [9]: Genes up-regulated in either 1) in vitro tachyzoites or 2) in vitro bradyzoites were each placed into their own gene sets. Genes up...
Consistent with other studies using the FUCCI reporter10,13,34,35,36,37,38,39,40, we observed that mCherry accumulates after cell division during G1 and is lost during the G1/S transition as mVenus is expressed (Supplemental Movie 2). We also performed DAPI staining to evaluate the DNA...
consisting of nine cell types that showed trait relevance at different levels, where 200 cells per labeled cell type were synthesized. SCAVENGE was applied to these simulated datasets using the default parameters, except for evaluation of the number of seed cells and the number of neighbors used ...
Even though in OncM mice the T cells appear to go through the same developmental stages as regular thymocytes, they are less functional, which may be the result of positive selection by other hematopoietic cells instead of thymic epithelium [25]. This, however, remains to be elucidated for T...