SPIKE PROTEINMOUSE MODELNANOPARTICLESThe response by vaccine developers to the COVID-19 pandemic has been extraordinary with effective vaccines authorized for emergency use in the United States within 1 year of the appearance of the first COVID-19 cases. However, the emergence of SARS-CoV-2 ...
responses generated against major protein antigens, of parasitic (Schistosoma mansoni) and bacterial (Clostridium tetani) origin, present characteristic Th ... EE Comoy,A Capron,G Thyphronitis - 《International Immunology》 被引量: 167发表: 1997年 ...
The nucleotide sequence of the region 5' to the fkbA gene, encoding the Streptomyces chrysomallus FK506 binding protein (FKBP-12), revealed an open reading... A,Pahl,U,... - 《Embo Journal》 被引量: 34发表: 1994年 Reclassification of Streptomyces nigrifaciens as a later synonym of Stre...
We show that during phage infection, this system specifically conjugates the ubiquitin-like protein to the phage central tail fibre, a protein at the tip of the tail that is essential for tail assembly as well as for recognition of the target host receptor. Following infection, cells encoding ...
Polymerase chain reactions were used to insert different ZIKV B-cell epitopes (representing aa 241–259, 294–315, 317–327, 346–361, 377–388 and 421–437 from the envelope protein of ZIKV strain MR-766; Fig. 1A) Two ZIKV B-cell epitopes inserted on the coat proteins of MS2 and PP...
Phage-like protein-translocation structures represent a third group of contractile phage tail-like particles and resemble partly T6SSs and partly R-type tailocins The terminology of contractile phage tail-like particles (CPTPs) is confusing and misleading CPTPs are manifold and affect all domains of...
The experiments described here are aimed at satisfying the first of these two requirements by engineering efficient peptide display at two different sites in MS2 coat protein. First, we evaluated the suitability of the N-terminus of MS2 coat for peptide insertions. It was observed that random N-...
Protein sequence-based analysis of the p-crAssphage demonstrated that this phage is the founder member of a family-level group of ‘crAss-like phages’ and was predicted to have a podovirus-like morphology [18]. We have proposed a taxonomic system for crAss-like phages from the human gut ...
Then, the fragment of maturase and single-chain version of the coat protein dimer containing the His-tag was cloned into the multiple cloning site 1 (MCS1) of the pACYCDuet-1-TM to create the pACYCDuet-1-TM-CoatDimer-His vector. Full size image Construction of pACYCDuet-1-TM-CoatDimer...
The gene products involved in mammalian mitochondrial DNA (mtDNA) maintenance and organization remain largely unknown. We report here a novel mitochondrial protein, Twinkle, with structural similarity to phage T7 gene 4 primase/helicase and other hexamer