phage, located on the sidewall of filamentous phage. pVIII protein is most commonly used due to its high copy number, but it can only tolerate a small peptide (about 10 amino acids) [118] as it must pass through the cell membrane, and only when the helper phage provides the pVIII prot...
For a subset, we determined their receptors using efficiency of plating (EoP) assays on two broadly sensitive strains, their deep-core (∆rfaD) lipopolysaccharide (LPS) mutant derivatives and their surface protein knock-out mutants (Tsx, LamB, OmpC, OmpA, TolC and OmpF) from thereof (...
Our understanding of the molecular mechanisms behind bacteria-phage interactions remains limited. Here we report that a small protein, SrpA, controls core cellular processes in response to phage infection and environmental signals inPseudomonas aeruginosa. We show that SrpA is essential for efficient geno...
of the downstream o-spanin gene. This would need to be followed by internal deletions within the non-overlapping region in the i-spanin to restore the overall length. The intermediate i-spanin in this pathway could easily be fully functional, since the addition of a few random residues at ...
Fig. 2. Gene order of T4 core genes and nearly universal core genes in ΦM12 compared with the most closely-related cyanophages (based on the number of core protein sequence best matches) Syn1; S-SM2; Syn9; P-SSM4; P-HM1; and with Pelagibacter phage HTVC008M. The gene order of...
Fig. 3. Basic structure of MS2 nanocarriers: A) the external surface of the MS2 coat displays the modified amino acid p-aminophenylalanine (paF19) and the protein on the internal phage surface displays cysteine residues (Cys 87). B) After removing the phage genome, the Cys 87 is modifi...
In silico, secretome proteins can be predicted from completely sequenced genomes using various available algorithms that identify membrane-targeting sequences. For metasecretome (collection of surface, secreted and transmembrane proteins from environment
and encode three core proteins necessary for excision and replication. PICMIs are dependent on virulent phage particles to spread to other bacteria, and protect their hosts from other competitive phages without interfering with their helper phage. Thus, our work broadens our understanding of phage sat...
Here we report that a small protein, SrpA, controls core cellular processes in response to phage infection and environmental signals in. We show that SrpA is essential for efficient genome replication of phage K5, and controls transcription by binding to a palindromic sequence upstream of the ...
The luxAB expression cassette may be cloned into an internal restriction site, and be flanked by the φA1122 DNA to create pluxABACYC184 (FIG. 2). FIG. 2 illustrates a schematic of a Yersinia shuttle vector and homologous recombination process based on a double crossover event. In FIG. 2...