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Surface, secreted and transmembrane protein-encoding open reading frames, collectively the secretome, can be identified in bacterial genome sequences using bioinformatics. However, functional analysis of translated secretomes is possible only if many sec
Isolation of phages for phage therapy: a comparison of Spot tests and efficiency of plating analyses for determination of host range and efficacy. Volume 10. PLOS ONE. Public Library of Science; 2015. p. e0118557. Kutter E, Sulakvelidze A. BACTERIOPHAGES: BIOLOGY AND APPLICATIONS.:5. ...
Kutter E. Phage host range and efficiency of plating. Methods Mol Biol. 2009;501:141–9. Article CAS PubMed Google Scholar O’Flaherty S, Coffey A, Edwards R, Meaney W, Fitzgerald GF, Ross RP. Genome of staphylococcal phage K: a new lineage of myoviridae infecting gram-positive bacter...
The phage cocktails of P.aeruginosa phages was isolated that had extended host range activity against all MDR, XDR, and PDR strains. According to TEM data, three bacteriophages belonging to Cystoviridae , Leviviridae , and Inoviridae families were identified. Phage cocktails used in this study ...
However, acquisition of resistance by bacteria is a major issue in the successful development of phage therapies. Through natural evolution and structural modeling, we identified host-range determining regions (HRDR) in the T3 phage tail fiber protein and developed a high-throughput strategy to ...
5) host range mutant 寄主范围突变型6) host-phage 寄宿主-噬菌体 1. Analysis on the stability of host-phage dynamics model 寄宿主-噬菌体动力学模型的稳定性分析 更多例句>> 补充资料:操纵基因突变体 分子式:CAS号:性质:因操纵子的操纵基因突变而导致调节蛋白无法与之结合的细菌突变体。其特点是操纵...
P. putida phages represented in the bottom panel (E) phage A8 (F) phage A9 (G) phage A10 and (H) phage A11. Full size image Phage host range determination and efficiency of plating (EOP) Spot test assays were conducted to determine the host range of each phage isolated (Table S13)....
For a subset, we determined their receptors using efficiency of plating (EoP) assays on two broadly sensitive strains, their deep-core (∆rfaD) lipopolysaccharide (LPS) mutant derivatives and their surface protein knock-out mutants (Tsx, LamB, OmpC, OmpA, TolC and OmpF) from thereof (...