weclonedmousePGC-1apromotor(-2533-+78)andconstructedthe30pGL3-PGC-1a-Lucreportervectoranditsmutants.ThetranscriptionactivitywasanalyzedbyLuciferaseassay.PCRwasusedtoobtainDNAfragmentsthatcontainthepromoterofPGC-1agenebyusinggenomeDNApreparedfrommouseliverasthetemplates.ReporterplasmidspGL3-PGC-1a-Lucwhichcontain...
20658-1-AP targets PGC1a in WB, IF, IHC, chIP, ELISA applications and shows reactivity with human, mouse samples. Tested Applications ELISA Application Description Cited Applications WB, IF, IHC, chIP Tested Reactivity human, mouse Cited Reactivity human, mouse, rat, goat, chicken, zebrafish ...
We used gain- and loss-of-function models in mouse primary hepatocytes and measured hepatocyte insulin response by gene and protein expression and ex vivo glucose production. We found that the PGC1A level determines the relative ratio of IRS1 and IRS2 in hepatocytes, impacting insulin receptor ...
Therefore, the aim of this study was to generate a monocistronic lentiviral vector expressing PGC to investigate whether this therapy could ameliorate the cognitive deficit ofAPP23 mouse model of AD at an advanced stage of pathology. A recombinant lentiviral vector carrying human codon optimized PGC...
Immunofluorescent analysis of (4% PFA) fixed HeLa cells using PGC1a antibody (66369-1-Ig, Clone: 1C1B2 ) at dilution of 1:2000 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Mouse Recombinant Secondary Antibody (H+L) (RGAM002). ...
Proteintech's Mouse Monoclonal PGC1a antibody is validated in IF/ICC, FC (Intra) and shows reactivity with human, mouse, rat samples.
浓度:2000 μg/ml 库存:现货 Size 点击咨询在线客服 WB Autoantibodies against the NineTeen complex and U5 RNP in systemic sclerosis mouseWB Nat Commun Imprinted lncRNA Dio3os preprograms intergenerational brown fat development and obesity resistance. ...
Overexpression of the peroxisome proliferator-activated recep- tor g co-activator 1a (PGC-1a) in cultured muscle cells is sufficient to promote postsynaptic NMJ gene transcription and the formation of aneural AChR clusters. Moreover, skeletal muscle-specific gain- and loss-of-function mouse models ...
Furthermore, FOXO1 function is required for the robust activation of gluconeogenic gene expression in hepatic cells and in mouse liver by PGC-lα. Insulin suppresses gluconeogenesis stimulated by PGC-lα but coexpression of a mutant allele of FOXO1 insensitive to insulin completely reverses this ...