Proteins were resolved by SDS-PAGE, transferred to a polyvinylidene difluoride membrane and incubated with an anti-synaptophysin antibody (Abcam, Ref. ab53166, 45 kD), anti-SCN4A antibody (Novus Biological, Ref NBP1–19008, 208 kDa) and SV2A antibody (Novus Biological, Ref NBP1-46367, 88 ...