Cell extract was prepared and enzyme activity determined using an assay kit (ab109909) from Abcam (Cambridge, MA, USA) according to the manufacturer's protocol. The amount of lysate used was 15 mg for A549 and 30 mg for H460. The results were further expressed as relative level with ...
(mouse, 1:500, Molecular Probes), cytochrome C (mouse, 1:1,000, BD Parmingen), TFAM (rabbit, 1:500, Abcam), NRF1 (rabbit, 1:500, Life Span Bioscience) and, p-CREB,CREB p-AKT, p-ERKs and ERKS (rabbit, 1:1,000, Cell Signaling Technology), PSD95 (mouse, 1:500, Thermo ...
Proteins were resolved by SDS-PAGE, transferred to a polyvinylidene difluoride membrane and incubated with an anti-synaptophysin antibody (Abcam, Ref. ab53166, 45 kD), anti-SCN4A antibody (Novus Biological, Ref NBP1–19008, 208 kDa) and SV2A antibody (Novus Biological, Ref NBP1-46367, 88 ...
MC3T3-E1 cells were cultured for 7 d in osteogenic medium under 1 g, SMG or SMG + CNF1 culture conditions to assay ALP activity. Cells were washed twice with PBS and lysed for 30 min on ice in RIPA buffer. ALP activity was measured with an ALP Assay Kit (Abcam, Cambridge, MA, USA...
MC3T3-E1 cells were cultured for 7 d in osteogenic medium under 1 g, SMG or SMG + CNF1 culture conditions to assay ALP activity. Cells were washed twice with PBS and lysed for 30 min on ice in RIPA buffer. ALP activity was measured with an ALP Assay Kit (Abcam, Cambridge, MA, USA...