Full size image The BMAL1/SIRT1/PGC-1α pathways and mitophagy damage are inhibited in HG induced podocytes injury To examine whether BMAL1 and SIRT1/PGC-1α pathways are involved in HG-induced DN, levels of these factors in MPC5 (control) and HG-treated MPC5 (HG group) were determined...
The results of our study are presented in the form of a ratio of the intensity, which is calculated by dividing the intensity of the target protein bands by the intensity of the GAPDH band. Statistics Statistical analyses were performed by using SPSS Version 25.0 (Chicago, IL, USA). ...
Full size image PGC-1α orchestrates the release of mtDNA, oxidative stress, and TNFAIP3 to regulate NLRP3 inflammasome To further clarify the mechanistic link between PGC-1α and NLRP3 inflammasome pathway in kidney injury, we first examined the changes in mtDNA upon cell injury, which is kno...
Full size image Melanoma cells induce mitochondrial biogenesis in MSC We and others have shown that mitochondria can be transferred from non-malignant to malignant cells [22, 24, 26, 46,47,48,49,50,51,52,53]. Next, we wanted to assess the influence of melanoma cells on mitochondrial biogen...
Full size image To assess the impact of VML on the mitochondrial structure across the entire muscle, we imaged a VML-injured muscle 7 days after injury at various distances (i.e., 0, 0.5, 1.5, 2.0 and 2.5 mm) from the border of the injury in the proximal direction toward the origin...
School of Life Sciences, Nanjing University, Nanjing 210093, China; 2Tianjin Medical University Cancer Institute and Hospital, Tianjin 300060, China Peroxisome proliferator-activated receptor gamma (PPARγ) coactivator-1 alpha (PGC-1α) coactivates multiple transcription factors and regulates several metab...
Full size image We compared the metabolite profiles of cells treated with LPS or IFN-β using LC–MS (Fig. 1b) and analysis of genes encoding factors involved in metabolic pathways (Fig. 1c). LPS induced significant changes in metabolites involved in glycolysis, the TCA cycle, pentose phosph...
Cell transfection Full-length sequences, including circHIPK3, SIRT1 and mitofusin 2 (MFN2), were amplified and cloned into pcDNA3.1 vector to construct circHIPK3, SIRT1 and MFN2 overexpression vectors. The siRNA negative con- trol (si-NC), siSIRT1 and si-peroxisome proliferator- activated...
Full size image Using qRT-PCR, we identified the expression ofBcl-2, SIRT1, PGC-1α, andBaxmRNA in the mouse hippocampus samples. The expression trends were consistent with the western blot findings: theBcl-2/Bax(mRNA) ratio in the Aβ group was lower than that in the control and Aβ...
(ATA). This leads to 1% translation efficiency compared to FNDC5 with canonical ATG in vitro. However, Albrecht et al. have shown in the new study that the first exon with the mutant codon is not expressed at all by RNA-seq. This prevents even the theoretical opportunity to produce full...