gRNA依赖polyA。 由于盒大小的限制,CROP-seq被认为与多种sgRNA的递送不兼容。 第一批应用:Perturb-seq: Dissecting molecular circuits with scalable single cell RNA profiling of pooled genetic screens - 2017 - Cell 第一代其他: Dissecting Immune Circuits by Linking CRISPR-Pooled Screens with Single-Cell ...