Homology modeling and docking studies of Peroxisomal carnitine O-octanoyltransferasedoi:10.21608/dusj.2022.233891DRUG designMOLECULAR dockingTRANSFERASESHOMOLOGY (Biochemistry)ENZYMESComputational docking is used for optimizing known drugs and for defining new binders by simulating their binding m...
This notion is supported further by the presence of carnitine octanoyl transferase in peroxisomes [22], [23] providing a carnitine dependent mechanism for export of medium chain fatty acid generated in mammalian peroxisomes. Disruption of Acox1 in mice results in infertility, retarded postnatal growth...
chlorophenyl)pentyl) oxiran-2-carbox- ylic acid Experiments with chlorpromazine, an inhibitor of peroxisomal carnitine octanoyltransferase, have led to the proposal that per- oxisomal ,B-oxidati on which was depressed by the phen othiazine in isolated hepatocytes (1) was dependent on carnitine (...
The enzymes involved: 1, carnitine acetyltransferase; 2, carnitine octanoyltransferase. The possible role of peroxisomal thioesterases in the transfer of acetyl/acyl groups out of peroxisomes has been discussed elsewhere (Hiltunen et al., in this issue). Reduction of NADP+ in peroxisomes is ...
Home > Section > Chapter Frenkel, R.A.; Slaughter, C.A.; Orth, K.; Moomaw, C.R.; Hicks, S.H.; Snyder, J.M.; Bennett, M.; Prough, R.A.; Putnam, R.S.; Milewich, L. Journal of Steroid Biochemistry 35(2): 333-342 ...
G. (2000) Identification of the two histidine residues responsible for the inhibition by malonyl-CoA in peroxisomal carnitine octanoyltransferase from rat liver. FEBS Lett. 466, 183-186Morillas, M., Clotet, J., Rubi!, B., Serra, D., Asins, G., Arin4 o, J. and Hegardt, F. G. (...
Abbreviations : Acox1 : acyl-Coenzyme A oxidase 1, palmitoyl; Acot : acyl-CoA thioesterase; ACAA: acetyl-Coenzyme A acyltransferase; ABCD3/PMP70: ATP-binding cassette, sub-family D (ALD), member 3; BafA1: bafilomycin A1; Crot : carnitine O-octanoyltransferase; CTSB: cathepsin B; Decr2 ...
Molecular cloning and expression of human carnitine octanoyltransferase: evidence for its role in the peroxisomal β-oxidation of branched-chain fatty acids Biochem. Biophys. Res. Commun., 263 (1999), pp. 213-218 View in ScopusGoogle Scholar [32] S.J. Steinberg, S.J. Wang, D.G. Kim, ...
Carnitine moiety is then transferred to the acyl-CoA or the acetyl-CoA by carnitine octanoyltransferase (CROT) or carnitine acetyltransferase (CRAT). On the other hand, a specific peroxisomal thioesterase can hydrolyze the acyl-CoA or the acetyl-CoA, giving a free fatty acid or acetate that ...
(vi) 0.5 μM carbonyl cyanide m-chlorophenyl hydrazone (CCCP; Sigma-Aldrich, C2759), (vii) 10 mM glutamate (Sigma-Aldrich, G1626), (viii) 50 mM succinate (Sigma-Aldrich, S2378), (ix) 0.5 mM octanoylcarnitine (BioTrend, Cologne, Germany; B6371), and (x) 0.5 μM rotenone (Sigma-...