aIllustration of the pathological process of pre-metastatic niche (PMN) formation. The primary tumor produces pro-metastatic factors, such as tumor-derived secreted factors (TDSF), to induce fibroblast activation in metastatic destination organs. The tumor-educated activated fibroblasts serve to construc...
The gel was incubated for 15 min in depurination buffer (0.25 M HCl), rinsed with ddH2O and incubated with 0.5 M NaOH for 30 min, rinsed with ddH2O and incubated in 0.5 M NaOH and 1.5 M NaCl for 30 min, and finally rinsed with ddH2O and incubated in 1 M ...
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The use of caustic soda (NaOH), which is a low-cost product, to obtain useful peptides from porcine haemoglobin is studied in this paper. Concentrations of 6MNaOH at 50 degrees C for 24 h afforded an 80% peptide recovery yield with an average peptide size of 13 kDa. Product obtained ...
Stock solutions of peptides were prepared by dissolving 0.85 mg and 0.94 mg of h5e (MW 1370.6951) (see Table 3 below) and h9e (MW 1740.9167), respectively, in 500 μL HPLC grade water and adding 60 to 80 μL of 0.25 M NaOH to obtain solutions with a pH of 8. Solutions for MS...
[39,40,55]. As a further chemical functionalization, 32.3% nitric acid (immersion time 10 min) can be applied to tantalum [51]. Alkaline solutions such as NaOH could also be used for introducing hydroxyl groups. Notable examples include immersion of magnesium AZ 31 at 80 °C for 12 h [...
Briefly, 24 mL of collagen solution was neutralized with 6 ml of 10 × phosphate buffered saline (PBS) and 4 mL of 0.1 N NaOH solution in an ice bath. The mixed solution was loaded into a 96-well plate with 300 μL in each well and incubated at 30 °C for 3 days. Reconstituted ...
Endogenous peroxidase activity was quenched with a 30 min incubation in 0.3% H2O2 in water, and sections microwaved for 5 min in antigen retrieval solution (0.01 M citric acid, 0.05 M NaOH pH 6.0). Sections were blocked with 2% goat serum in PBS then incubated with primary antibody (either...
双缩脲反应 NH2-CO-NH-CO-NH2 ( 双缩脲),能与 CuSO4-NaOH产生颜色反应。 凡是有肽键结构的化合物都会发生双缩 脲反应,可用于定量分析。 双缩脲反应是肽和蛋白质特有的反应, 游离氨基酸无此反应。 四、天然存在的活性肽 1、 谷胱甘肽(GSH) 三肽(Glu-Cys-Gly),广泛存在于生物细胞中,含 有自由的巯基,...
4b). 10 can be hydrolysed in 0.1 N NaOH to yield 11. Production of 10 and 11 indicated that the biosynthetic process stopped after the formation of the monomer. The same products were observed from the reaction of purified C-His6-tagged BbBEAS-H2901A (trace iv, Fig. 4b), ...