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4b). 10 can be hydrolysed in 0.1 N NaOH to yield 11. Production of 10 and 11 indicated that the biosynthetic process stopped after the formation of the monomer. The same products were observed from the reaction of purified C-His6-tagged BbBEAS-H2901A (trace iv, Fig. 4b), ...
aIllustration of the pathological process of pre-metastatic niche (PMN) formation. The primary tumor produces pro-metastatic factors, such as tumor-derived secreted factors (TDSF), to induce fibroblast activation in metastatic destination organs. The tumor-educated activated fibroblasts serve to construc...
Geriatr Gerontol Int 2016; : -. 2016 Japan Geriatrics Society.doi:10.1111/ggi.12951Hamagawa, KosukeKubo, ToruNishimura, KotaroBaba, YuichiHirota, TakayoshiYamasaki, NaohitoKitaoka, HiroakiGeriatrics & Gerontology International
英文名称:Unsubstituted Resins for Solid Phase Synthesis Hydroxymethylbenzoyl-MBHA Resin Divinylbenzene 1%, 200-400 mesh For preparation of various C-modified peptides (amides, hydrazides, alcohols, methyl esters or acids) Reagent: NH3/MeOH, NH2NH2/DMF, NaOH (aq), MeOH/Et3N, NaBH4/EtOH Product...
The microalgae biomass was suspended in 1 mL of distilled water and subsequently 2 mol L−1 of NaOH was added to water solution to adjust the sample pH at 11 and protein extraction was carried out under continuous stirring for 15 min and at room temperature (RT). The resulting mixture ...
[39,40,55]. As a further chemical functionalization, 32.3% nitric acid (immersion time 10 min) can be applied to tantalum [51]. Alkaline solutions such as NaOH could also be used for introducing hydroxyl groups. Notable examples include immersion of magnesium AZ 31 at 80 °C for 12 h [...
Endogenous peroxidase activity was quenched with a 30 min incubation in 0.3% H2O2 in water, and sections microwaved for 5 min in antigen retrieval solution (0.01 M citric acid, 0.05 M NaOH pH 6.0). Sections were blocked with 2% goat serum in PBS then incubated with primary antibody (either...
DMPC and 2:1 DMPC:DMPG (w/w) were dissolved in chloroform and methanol (3:1 v/v) at 10 mg/mL, dried using a rotary evaporator and left 3 hours under high vacuum in a freeze dryer. Phospholipids were then dissolved in 20 mM Sodium phosphate – NaOH, 150 mM NaCl, pH ...
The gel was incubated for 15 min in depurination buffer (0.25 M HCl), rinsed with ddH2O and incubated with 0.5 M NaOH for 30 min, rinsed with ddH2O and incubated in 0.5 M NaOH and 1.5 M NaCl for 30 min, and finally rinsed with ddH2O and incubated in 1 M ...