This research frequently involves the KC (LSL:KrasG12D;Pdx1-CRE) genetically engineered mouse model, which leads to pancreatic cancer predisposition. However, as frequently encountered in animal models, the KC mouse model also exhibits biases. Herein, we report a new adverse effect of KrasG12D...
将2A-Cre插入到小鼠Pdx1基因终止密码子处。 *使用本品系发表的文献需注明: Pdx1-2A-Cre mice (Cat. NO. NM-KI-225082) were purchased from Shanghai Model Organisms Center, Inc.. 验证数据 Fig1. Cre mediated recombination in pancreasof Pdx1Cre/+;Rosa26tdTomato/+mice. ...
In addition, body weight records and a glucose tolerance test revealed no differences between WT and PAK4 KO mice. Together, this suggests that PAK4 is dispensable for mouse pancreas development. This will facilitate future use of our Pdx1-Cre-driven conditional PAK4 KO mouse model for testing...
(α-SMA) expression. CCG-222740 also modulates inflammatory components of the pancreas in KC mice (LSL-KrasG12D/+; Pdx-1-Cre) stimulated with caerulein. It decreases the infiltration of macrophages and increases CD4 T cells and B cells. Analysis of the pancreatic adenocarcinoma (PDA) TCGA ...
*使用本品系发表的文献需注明: Pdx1-2A-Cre mice (Cat. NO. NM-KI-225082) were purchased from Shanghai Model Organisms Center, Inc.. 验证数据 Fig1. Cre mediated recombination in pancreasof Pdx1Cre/+;Rosa26tdTomato/+ mice. The white dashed line indicates the islets of langerhans. 上海南方...
To study the late beta-cell-specific function of the homeodomain protein IPF1/PDX1 we have generated mice in which the Ipf1/Pdx1 gene has been disrupted sp... R Katsuse,坂本,昭一郎,大村,栄一,... - 《Genes & Development》 被引量: 344发表: 1998年 SOX6 Attenuates Glucose-stimulated ...
Pdx1-BFP mice are viable and fertile without signs of MODY4, and BFP fluorescence reflects endogenous PDX1 levels26. To allow identification of non- β-cells, Ins1CreThor animals were crossed with the R26mTmG reporter strain (IMSR Cat# JAX:007576, RRID:IMSR_JAX:007576), resulting in Ins...
Conditional gene targeting in mouse pancreatic ß-Cells: analysis of ectopic Cre transgene expression in the brain Diabetes, 59 (2010), pp. 3090-3098 Crossref View in Scopus Google Scholar Wilcox et al., 2013 C.L. Wilcox, N.A. Terry, E.R. Walp, R.A. Lee, C.L. May ...
Experiments were performed on islets isolated from 8 to 10-week-old mice housed in a 12hr light/dark cycle with ad libitum access to food. The University of Pennsylvania Institutional Animal Care and Use Committee approved all mouse studies. 2.2. Cell culture and lentiviral infection P20-30 ...
To study the in vivo role of SG formation in pancreatic function, Tia1f/f mice were crossed with Ins2Cre mice to generate beta cell-specific Tia1-knockout mice (ESM Fig. 1). BetaTIA1-KO mice were viable, fertile and indistinguishable from the WT littermates. We confirmed the pancreatic...