KC miceThe highly utilized KC model has a reported lethality rate of about 30%, which has been attributed to pancreas cancer. However, a competing cause of lethality in KC mice is due to the activation of mutant- Kras gene ( Kras G12D/+ ) in the multipotent progenitor cells (MPP), ...
将2A-Cre插入到小鼠Pdx1基因终止密码子处。 *使用本品系发表的文献需注明: Pdx1-2A-Cre mice (Cat. NO. NM-KI-225082) were purchased from Shanghai Model Organisms Center, Inc.. 验证数据 Fig1. Cre mediated recombination in pancreasof Pdx1Cre/+;Rosa26tdTomato/+mice. ...
In addition, body weight records and a glucose tolerance test revealed no differences between WT and PAK4 KO mice. Together, this suggests that PAK4 is dispensable for mouse pancreas development. This will facilitate future use of our Pdx1-Cre-driven conditional PAK4 KO mouse model for testing...
*使用本品系发表的文献需注明: Pdx1-2A-Cre mice (Cat. NO. NM-KI-225082) were purchased from Shanghai Model Organisms Center, Inc.. 验证数据 Fig1. Cre mediated recombination in pancreasof Pdx1Cre/+;Rosa26tdTomato/+ mice. The white dashed line indicates the islets of langerhans. 上海南方...
(α-SMA) expression. CCG-222740 also modulates inflammatory components of the pancreas in KC mice (LSL-KrasG12D/+; Pdx-1-Cre) stimulated with caerulein. It decreases the infiltration of macrophages and increases CD4 T cells and B cells. Analysis of the pancreatic adenocarcinoma (PDA) TCGA ...
To study the late beta-cell-specific function of the homeodomain protein IPF1/PDX1 we have generated mice in which the Ipf1/Pdx1 gene has been disrupted sp... R Katsuse,坂本,昭一郎,大村,栄一,... - 《Genes & Development》 被引量: 344发表: 1998年 SOX6 Attenuates Glucose-stimulated ...
Pdx1-BFP mice are viable and fertile without signs of MODY4, and BFP fluorescence reflects endogenous PDX1 levels26. To allow identification of non- β-cells, Ins1CreThor animals were crossed with the R26mTmG reporter strain (IMSR Cat# JAX:007576, RRID:IMSR_JAX:007576), resulting in Ins...
Conditional gene targeting in mouse pancreatic ß-Cells: analysis of ectopic Cre transgene expression in the brain Diabetes, 59 (2010), pp. 3090-3098 Crossref View in Scopus Google Scholar Wilcox et al., 2013 C.L. Wilcox, N.A. Terry, E.R. Walp, R.A. Lee, C.L. May ...
1. To biuld up Ptf1a & Pdx1 double gene lineage tracing in mice model and gene identification. (1) Propagating and screening Pdx1-Dre\Ptf-1a-Cre Knock in mice as well as Dre & Cre reporter mice of Rosa26CAG-RSR-AcGFP1 or CAG-LSL-Tomato. (2) Time mating to get the lineage...
Experiments were performed on islets isolated from 8 to 10-week-old mice housed in a 12hr light/dark cycle with ad libitum access to food. The University of Pennsylvania Institutional Animal Care and Use Committee approved all mouse studies. 2.2. Cell culture and lentiviral infection P20-30 ...