它们在CD39+细胞中的比例较低，有利于IFN-γ单阳性细胞，表明末端衰竭(图3A)。对IFN-γ+和TNF+群体的分析表明，PD-1hiCD39+细胞中各自的MFI均低于其他所有群体(图3B)，这表明这些细胞产生的细胞因子数量更少。根据PD-1hiCD39+细胞中TIM-3的表达情况，PD-1hiCD39+细胞及其MFI没有发生变化(图3,A和B)。...
The mean fluorescent intensity (MFI) was quantified and normalized against background signal. Results Our results show a significant increase in PD1 expression in inflammatory cells in dysplasia and OSCC compared to hyperkeratosis. PDL1 expression in epithelial cells was significantly increased in OSCC ...
+ 和pd-l1-细胞,以通过流式细胞术测量ifn-γ的mfi,如图11d所示。图11e示出了在经il-12加il-18(每种细胞因子为10 ng/ml)处理的并且经或未经10 μg/ml的ifn-γ受体1中和mab(αifn-γr1 nab)、10 μg/ml的ifn-γ受体2中和mab(αifn-γr2 nab)或者αifn-γr1 nab和αifn-γr2 nab的组合(每种为...
而后每孔加100μl的pbs重悬,吹匀细胞后上机检测。使用bd公司cantoii型号流式细胞仪测定每孔细胞的平均荧光强度mfi值。使用graphpadprism软件进行数据处理,得出抗体结合细胞的ec50浓度值(即达到该抗体最大荧光结合信号50%时对应的抗体浓度值),结果如图3。从图中可以看出,本发明的d6a2c2b2单克隆抗体可特异性结合细胞膜...
PD-L1 expression in patient tissues was analyzed using RK-10-Cy5 and compared to expression in MDA-MB-231 cells and a negative control of normal blood (Fig.5). The MFI of the MDA-MB-231 cells was 9,448, while the whole blood gave an MFI of −123 (S9). Squamous cell carcinoma ...
使用BD公 司Canto II型号流式细胞仪测定每孔细胞的平均荧光强度MFI值。使用GraphPad Prism软 8 8 CN 113150153 B 说明书 7/11 页 件进行数据处理,得出抗体阻断PD1/PDL1结合的IC50浓度值,结果如图6。从图中可以看出, 本发明的D6A2C2B2单克隆抗体可特异性阻断人PD1蛋白结合细胞膜表面的人PDL1配体,其 阻断能力...
(95% confidence interval: 0.61-0.99) for CD8+CD38+ MFI, 0.96 (95% confidence interval: 0.85-0.99) for CD8β infiltration, and 0.97 (95% confidence interval: 0.87-0.99) for CK+HLAabc+ cell rate. Receiver operating characteristic curve analysis for predicting recurrence showed an area under ...
Habicht ADada SJurewicz MFife BTYagita HAzuma MSayegh MHGuleria IThe Journal of Immunology: Official Journal of the American Association of ImmunologistsHabicht A, Dada S, Jurewicz M, Fife BT, Yagita H, Azuma M, Sayegh MH, Guleria I. A link between PDL1 and T regulatory cells in feto...