Recombinant adenovirus Ad-PD-L1 had high efficiency expression of PD-L1 in recipient mouse. The blood glucose concentration of mice in the Ad-PD-L1 gene treatment group was obviously lower than that of the control and Ad-EGFP treatment groups and was stable and kept within the normal range ...
Davis for providing the mouse PD-L1 and PD-L2 with C-terminal biotin produced in CHO cells and Peter Steinberg for providing the NFκB::eGFP reporter Jurkat cells and T-cell stimulator cells (TSC). The MD simulations were supported by an NSF award (MCA08X014) in advanced computing ...
5133 (Human); 18566 (Mouse); 301626 (Rat); 100533201 (Porcine); 486213 (Canine); 102123659 (Cynomolgus Monkey) Alternate Names CD279 antigen; CD279; hPD-1; PD1; PD-1; PD1hPD-l; PDCD1; programmed cell death 1; programmed cell death protein 1; Protein PD-1; SLEB2 ...
ERT2;Pdcd1−/−mice on day 5 after tamoxifen injection. Max, maximum.f, Western blot analysis from lysates of ITK–SYK-expressing eGFP+T cells, sorted by flow cytometry fromITK-SYKCD4-CreERT2andITK-SYKCD4-CreERT2;Pdcd1−/−mice on day 5 after tamoxifen injection.g, ECAR metabolic...
5133 (Human); 18566 (Mouse); 301626 (Rat); 100533201 (Porcine); 486213 (Canine); 102123659 (Cynomolgus Monkey) Alternate Names CD279 antigen; CD279; hPD-1; PD1; PD-1; PD1hPD-l; PDCD1; programmed cell death 1; programmed cell death protein 1; Protein PD-1; SLEB2 ...
C2C12, 小鼠肌原细胞 Mouse 猪毒抗体(RV-ab)ELISA试剂盒96T/48T试剂盒 小鼠骨髓间充质干细胞(EGFP标记) 人小球组织糖基化终末产物(GTE-AGE)ELISA 试剂盒96T/48T试剂盒 HT-29(细胞) 5×106cells/瓶×2 羊睾酮(T)ELISA 试剂盒96T/48T试剂盒 使用方法: ...
to prepare the fluorescently labeled binding partner. PD-L1 or PD-1 protein is used as label-free binding partners. The KDvalue of 7.2 μM ± 1.9 μM between hPD-1 and hPD-L1-eGFP is obtained using MST [67], which is similar to SPR assay (KDvalue of hPD-1/hPD-L1 = ...
使制作的载体在CHOS细胞株中形质感染,以EGFP表达为基准,通过流 式细胞分析技术(flow cytometry)挑选生成人类PD‑1蛋白质细胞外域的细胞。于细胞培养 液中使用标签亲和层析法 (FLAG tag affinity chromatography)来对人类PD‑1蛋白质进 行纯化并定量化。 [0230] 细胞株抗原 [0231] 使包含人类PD‑1的cDNA的...
Bell (University of Chicago) for C57BL/6 MIP.eGFP mice; M. Nussenzweig (Rockefeller University) for C57BL/6 CD11c-YFP mice; A. Sharpe (Harvard Medical School) for C57BL/6 PD-L1-deficient mice; C. Benoist and D. Mathis (Harvard Medical School) for NOD BDC2.5 TCR–transgenic mice; R...
将绵羊pd-1-egfp表达cos-7细胞使用加入有10%钝化山羊血清(invitrogen公司)的pbs在室温下封闭15分钟,使10μg/ml的大鼠抗牛pd-1抗体5d2在室温下反应30分钟,清洗后使apc标记抗大鼠ig山羊抗体(beckman coulter公司)在室温下反应30分钟。作为阴性对照抗体,使用了大鼠igg2a(κ)同种型对照(bd biosciences公司)。在分析时...