1.3.2pd-l1蛋白的纯化与纯度鉴定收集培养基上清液,用ni-nta树脂纯化pd-l1-avi-his,随后用截留分子量为3kda的超滤离心管超滤浓缩pd-l1-avi-his,经sds-page验证其纯度,结果如附图2所示:其中,泳道1为蛋白ladder,泳道2为还原性pd-l1蛋白(+dtt)。泳道3为非还原性pd-l1蛋白(-dtt)。附图2表明:纯化后的pd-l1...
pd～Ni合金电镀周光月陈志全(贵金属研究所,昆明)摘要Pd—Ni合佥电键液系氨一氯化物体系。镀液中由于使用了光亮荆和润湿剂,舍佥键层白亮、镀层结晶细、孔隙少,耐磨损,抗腐蚀,有较低的接触电阻。镀液最佳pH=7.5～9,Hi浓度是7～1lg/L保证镀层中Pd的含量为70~80%。圭霉词铅镍台盒镀1.前言钯及钯合金是...
方法用Ni—NTA柱法纯化pd20一rNF一融合蛋白并进行Westernblotting检测;采用L929细胞毒法进行pd20一TNF—G/.融合蛋白的生物学活性检测;用流式细胞术检测不同浓度融合蛋白作用于胃癌肝高转移潜能细胞XGC9811一L的早期凋亡率,此实验分为5组:pd20一TNF一0【100.00、25.00、6.25U/m1分别为A、B、C组,不加融合蛋白的...
利用原核表达系统获得包涵体表达形式的重组蛋白h PD-L220~123(Ig V结构域)和h PD-L220~208(Ig V结构域和Ig C结构域);将重组蛋白利用Ni-NTA柱亲和层析纯化与复性后,作为免疫原免疫Balb/c雌性小鼠制备鼠抗人的多抗血清,经ELISA法检测其效价均达到1∶106;Western-blot实验结果表明,制备的多...
(PBST) buffer with the plate shaking at a speed of 1,000 rpm. Ni-NTA biosensors (Pall ForteBio) were first loaded with 10 μg ml−1PD-1 protein for 300 s and then associated with first MAbs(10 μg ml−1) for 600 s to get saturation. Afterwards, the biosensors...
Avni A novel plant cysteine protease has a dual function as a regulator of 1-aminocyclopropane-1-carboxylic acid synthase gene expression Plant Cell, 17 (2005), pp. 1205-1216 Google Scholar Miura et al., 2005 K. Miura, A. Rus, A. Sharkhuu, S. Yokoi, A.S. Karthikeyan, K.G. Rag...
SC505 (donor) labeled SNAP-PD-L1-His was pre-bound to LUVs via DGS-NTA-Ni. Subsequently added TMR (acceptor) labeled SNAP-CD80-His bound to the LUVs and interacted with PD-L1 in cis, causing FRET and SC505 quenching (black trace). On the right are time courses of normalized SC505 ...
Ni-NTA biosensors (Pall ForteBio) were first loaded with 10 mg ml À 1 PD-1 protein for 300 s and then associated with first MAbs(10 mg ml À 1) for 600 s to get saturation. Afterwards, the biosensors were dipped into the second MAb (10 mg ml À 1) in the presence of ...
5)ni-nta柱亲和层析纯化hpd-l2蛋白: a)取5mlni-nta填料置于玻璃柱中,自然沉降30min; b)利用30ml的ddh2o清洗柱子,然后用30ml平衡液(ph8.5,100mmtris-hcl,300mmnacl)平衡柱子,流速3s每滴; c)将待过柱的hpd-l2蛋白样品13000g,4℃离心15min,收集上清,0.45μm的滤膜进行过滤,待上样; ...
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