目前PCV3研究主要集中在流行病学和诊断方法等方面,而免疫机制及疫苗研发相关的研究报道较少。PCV3 ORF2基因编码的衣壳蛋白(Capsid protein, Cap),被广泛应用于PCV3致病机制的研究和基因工程疫苗的构建。虽然PCV2已有商品化的疫苗,但两种...
本领域的熟练技术人员可以将pcv2 orf2蛋白或衣壳蛋白表位延伸或添加到pcv3 orf2蛋白或衣壳蛋白中。在这方面,trible等人提到,“antibody recognition of porcine circovirus type 2capsidprotein epitopes after vaccination,infection and disease,clinical and vaccine immunology 18(5): 749-757(2011)doi:10.1128/cvi.0...
Capsid proteinAntibodiesIFAPorcine circovirus type 3 (PCV3) is a novel porcine circovirus associated with porcine dermatitis and nephritis syndrome (PDNS), reproductive failure, and multisystemic inflammation. Capsid protein (Cap) encoded by PCV3 ORF2 gene has been identified as an immunogenic ...
Based on the maximum likelihood mapping analysis of 303 PCV3 sequences a model with a combination of replicase, ORF3 and capsid protein coding genes was selected as the most appropriate target for genotyping, which provided the best support for the clade classification into three genotypes and ...
Result: In this study, we developed a real-time fluorescence PCR assay for the detection of PCV3. The conserved region within Capsid genome of PCV3 was selected for the design of primer pairs and probes. After optimizing, a primer pair and probe was screened, providing high sensitivity (10...
Capsid proteinAntibodiesIFAPorcine circovirus type 3(PCV3)is a novel porcine circovirus associated with porcine dermatitis and nephritis syndrome(PDNS),reproductive failure,and multisystemic inflammation.Capsid protein(Cap)encoded by PCV3 ORF2 gene has been identified as an immunogenic protein.Currently,...
Subunit vaccine of PCV3 capsid protein produced by sf9 cells with double knockout of Caspase-1 and Dronc induces strong immune response in miceSubunit vaccinePCV3CapSf9 cellsCRISPR-Cas9 systemKnockout sf9 cells aimed at Caspase-1 gene, Dronc gene and both genes were successfully generated by ...
Cap蛋白真核表达细胞凋亡细胞周期猪圆环病毒3型(Porcine Circovrius Type 3,PCV3)是一种新型猪病毒.其发病机制尚不清楚.PCVs无囊膜,具有唯一的结构蛋白—衣壳蛋白(Capsid protein,Cap).PCV2 Cap与病毒免疫原性和致病性等密切相关,提示PCV3 Cap在病毒入侵和致病性中亦应扮演重要的角色.目前对PCV3 Cap等ORFs编码...
In addition, sequencing of PCV3 and phylogenetic analysis were performed, based on a full genome and a capsid gene. In the current study, we demonstrated the high prevalence of PCV3 in the wild boar population in Poland; meanwhile, PCV4 was not detected. The odds of ASFV infect...
Virus-like particles (VLPs) of capsid protein (Cap) of PCV2 serve as an alternative nano-antigen delivery strategy to efficiently induce antiviral immune response against PCV2 and/or other covalently displayed swine pathogens. However, the current understanding is limited on the capability of PCV3...