DNA typing has revealed a high error rate for HLA-DR serology. Serological typing for HLA class I antigens is considered reliable, and it is generally believed that there is no need for the introduction of molecular methods for clinical HLA class I typing. In order to improve the ...
pcr-ssp法hla-a、b基因分型与血清学分型的比较 热度: 页数:4 单细胞PCR用于HLA分型的研究 热度: 页数:2 HLA基因分型和抗HLA抗体检测 热度: 页数:31 骨髓移植HLA基因分型程度探讨 热度: 页数:7 HLA 分型(PCR-SBT)法件应用介绍:HLA typing (PCR-SBT) method described application 热度: 页...
HLA-Dw特异性与HLA-DP特异性可分别通过纯合分型细胞(homozygotetypingcell,HTC)及预致敏淋巴细胞试验(primedlymphocytetest,PLT)检测。二种方法的基本原理均是判断淋巴细胞在识别非已HLA抗原决定簇后发生的增殖反应。由于分型细胞来源困难以及操作手续繁琐,细胞学分型技术下正逐渐淘汰。 三、HLA的DNA分型技术 1. 限制...
和重复性100%1宣论PcR—SSP检测HLA—DR的方祛具有快速,准确,特异性高等优点,适台临床应用 【关键词】聚台酶链反应人类自细胞抗原DR 中图号Q.pcR—r文献标识码A文章编号00x’oo002-oo3 DNATypingforHLA—?DRAntigensbyPolymcraseChainReactionwithSequence—— ...
HLA‐DR typing by PCR amplification with sequence‐specific primers (PCR‐SSP) in 2 hours: An alternative to serological DR typing in clinical practice inc... In most PCR-based tissue typing techniques the PCR amplification is followed by a post-amplification specificity step. In typing by PCR...
High-throughput DNA typing of HLA-A, -B, -C, and -DRB1 loci by a PCR–SSOP–Luminex method in the Japanese population We have developed a new high-throughput, high-resolution genotyping method for the detection of alleles at the human leukocyte antigen (HLA)-A, -B, -C, and... Y ...
Key words Sera-typing HLA-B locus PCR-SSP genotyping HLA(人类白细胞抗原)编码系统位于人类6号染色体短臂,主要有A,B,C,DR,DQ,DP等位点,其中与移植密切相关且作为临床配型指标主要有A,B,DR等[1]。B位点是其中最复杂,多态性最多的区域,其高变区各等位基因差异仅在几个碱基,且只有极少等位基因某个高变区...
综上所述,HLA-Ⅰ类基因分型PCR-SSP技术准确、特异,可作为造血干细胞移植配型和正常人群无关供者筛选的常规方法。 【参考文献】 1 Maduigal JA,Arguello R,Scott I,et al.Molecular histocompatibility typing in unrelated donor.one marrow transplantation.Blood Rev,1997,11(5):105-117. ...
In a recent study, we observed a discrepancy rate of 8.5% between the results of molecular and serological HLA class I typing in Caucasian kidney donors and recipients. In the present study we addressed the question how often black individuals are mistyped using the serological technique. 421 Bl...
【 Subject words 】 Polymorphism of HLA-A ARMS/PCR-SSP Gene typing 1991年第十一届国际HLA协作科研(11th WS)已正式明确HLA-Ⅱ类的DNA分型方法。本室于1993年报道了用PCR/SSO方法对湖南籍汉族人HLA-DR、DQ和DP座位多态性分析结果〔1〕。迄今,HLA-A、B和C的分型主要沿用血清学分型方法,即采用微量淋巴...