结论PCR-RSSO可识别HLA-Ⅰ,Ⅱ共706个等位基因,覆盖WHO命名委员会2000年公布的936个等位基因的75.43%;对706个HLA等位基因的分型均为中~高分辨率,有分辨纯合子等位基因的能力;易长期保存书面的实验原始资料,即杂交条;具有成本低,劳动强度低,省时和DNA消耗量少等优点.PCR-RSSO适合于造血干细胞移植和建立造血干细胞...
当前区分hla-c1/c2通常做法是:首先采用序列特异性引物-pcr(pcr-ssp)、基于luminex流式磁珠仪的反向序列特异性寡核苷酸探针杂交(pcr-rsso)或基于测序仪的pcr产物直接测序(pcr-sbt)的方法,鉴定并获得受检者的hla-c基因型;然后再在imgt/hla数据库中查看受检者中检出的hla-c等位基因的第80位密码子的序列及所编码的...
The results of KIR PCR-SSP, HLA PCR-rSSO and KIR3DL1 PCR-SBT, together with cervical cancer data from the TCGA database, were used to assess the association of iKIR genes, receptor-ligand gene combinations, iKIR transcription ... Jianxin,Zhen,Leilei,... - Zhonghua yi xue yi chuan xue...
Methods: Key inclusion criteria of this study was as follows: 20 years old and over, performance status 0 to 2, metastatic or recurrent CRC (adenocarcinoma), RAS wild-type CRC diagnosed by PCR-rSSO using tumor tissue, no prior use of anti-EGFR antibody. Plasma samples were prospectively ...
A novel PCR鈥搑everse sequence-specific oligonucleotide probe (PCR-rSSO) method-based mycobacterial detection panel (Myco-Panel) test was developed for the rapid identification of 30 mycobacterial species and subspecies. Clinical respiratory samples were collected from patients with suspected or confirmed...
The patient's molecular HLA typing was performed with rSSO. The laboratory obtained a frozen section of the renal allograft biopsy from 9/2012. DNA was extracted using a DNA tissue kit. Molecular HLA typing was performed using qPCR. Recipient and donor HLA phenotype results are presented in ...