(2010). Inter-laboratory validation of PCR-based protocol for detection of olive viruses. Bulletin OEPP/EPPO, 40 , 423–428.Loconsole G., Saponari M., Faggioli F., Albanese G., Bouyahia H., Elbeaino T., Materazzi A., Nuzzaci M., Prota V., Ro- manazzi G., Trisciuzzi N., ...
efficiency, a protocol was adopted to screen a microdissected B library with probes prepared from the degenerate oligonucleotide primed-PCR product of genomic... KL Lo,YP Lin,LJ Chen,... - 《Plant Molecular Biology Reporter》 被引量: 24发表: 2009年 Construction and use of spotted large-inser...
选中功能键 star t ar c h i vi n g 后,在目录 c : proguainm e ep p endorf r calp 1 exbacku P下建立一个档案文件.该过程将被记录在表lo 55、g中.4编程(检测项目得建立)master c ycler cp r e a Iplex 软件基本上包含有两个模块:as s ay set u p 与 analysi s。模块 assa y se ...
1.1 滴定分析法 滴定分析法先把未知浓度的CDNA 按一定比例稀释,同样把已知浓度CDNA 或参照也按比例稀释,在不同管内扩增得到合成DNA 的值,以起始CDNA 分子数N 0对合成DNA 分子数N 的对数LO G (N )作图,以此来估计不同样品中目标CDNA 的起始含量。1.2 动力学分析法 对于不同样品来说反映循环数n 决定了...
Cloning of large viral gene fragments for whole genome assembly Xie X, Lokugamage KG, Zhang X et al. (2021) Engineering SARS-CoV-2 using a reverse genetic system. Nat Protoc 16(3):1761–1784. Cloning of SARS-CoV-2 whole genome for functional studies Xie X,...
Shinohara ML, LoBuglio KF, Rogers SO: Group-I intron family in the nuclear ribosomal RNA small subunit genes of Cenococcum geophilum isolates. Curr Genet. 1996, 29: 377-387. 10.1007/s002940050059. Article CAS PubMed Google Scholar Egger KN, Osmond G, Goodier JL: Sequence and putative ...
buffer that is specially formulated with iso-stabilizing components. This unique buffer composition offers several advantages: No Tmcalculation for primers, more robust amplification of GC-rich target, enhanced amplification of long sequences, and auniversal PCR protocolfor high-throughput PCR...
Shinohara ML, LoBuglio KF, Rogers SO: Group-I intron family in the nuclear ribosomal RNA small subunit genes of Cenococcum geophilum isolates. Curr Genet. 1996, 29: 377-387. 10.1007/s002940050059. Article CAS PubMed Google Scholar Egger KN, Osmond G, Goodier JL: Sequence and putative ...
Cloning of large viral gene fragments for whole genome assembly Xie X, Lokugamage KG, Zhang X et al. (2021) Engineering SARS-CoV-2 using a reverse genetic system. Nat Protoc 16(3):1761–1784. Cloning of SARS-CoV-2 whole genome for functional studies Xie X, Mu...
12. Lo, C. L. et al. One-step rapid reverse transcription-PCR assay for detecting and typing dengue viruses with GC tail and induced fluorescence resonance energy transfer techniques for melting temperature and color multiplexing. Clinical chemistry 53, 594–599 (2007). 13. Bohling, S. D.,...