Shinohara ML, LoBuglio KF, Rogers SO: Group-I intron family in the nuclear ribosomal RNA small subunit genes of Cenococcum geophilum isolates. Curr Genet. 1996, 29: 377-387. 10.1007/s002940050059. Article CAS PubMed Google Scholar Egger KN, Osmond G, Goodier JL: Sequence and putative ...
Shinohara ML, LoBuglio KF, Rogers SO: Group-I intron family in the nuclear ribosomal RNA small subunit genes of Cenococcum geophilum isolates. Curr Genet. 1996, 29: 377-387. 10.1007/s002940050059. Article CAS PubMed Google Scholar Egger KN, Osmond G, Goodier JL: Sequence and putative ...
Cloning of large viral gene fragments for whole genome assembly Xie X, Lokugamage KG, Zhang X et al. (2021) Engineering SARS-CoV-2 using a reverse genetic system. Nat Protoc 16(3):1761–1784. Cloning of SARS-CoV-2 whole genome for functional studies Xie X, Muruato A, Lokugamage ...
buffer that is specially formulated with iso-stabilizing components. This unique buffer composition offers several advantages: No Tmcalculation for primers, more robust amplification of GC-rich target, enhanced amplification of long sequences, and auniversal PCR protocolfor high-throughput PCR...
About this protocol Cite this protocol Raeymaekers, L. (1998). Quantitative PCR. In: Lo, Y.M.D. (eds) Clinical Applications of PCR. Methods in Molecular Medicine™, vol 16. Humana Press. https://doi.org/10.1385/0-89603-499-2:27 Download citation .RIS .ENW .BIB DOIhttps://doi....
12. Lo, C. L. et al. One-step rapid reverse transcription-PCR assay for detecting and typing dengue viruses with GC tail and induced fluorescence resonance energy transfer techniques for melting temperature and color multiplexing. Clinical chemistry 53, 594–599 (2007). 13. Bohling, S. D.,...
PCR conditions and cycle protocol for the nested PCRs with the DGGE primer pairs were the same as those used for the PCRs with the group-specific primer pairs, except for the ATs which are shown in Table 2. DGGE of the PCR products was performed on a 8% (w/v) polyacrylamide gel ...
A one-step RT-PCR protocol and different primer sets, targeting the most common olive viruses covered by phytosanitary rules, were tested in each laboratory, using the same batch of positive and healthy controls as well as the same amplification conditions and reaction components. The one-step ...
设置程序,运行实验定量PCR软件操作基本步骤为:a.设置热循环程序文件(Protocol Tab ) b.设置反应板文件(Plate Tab )。C.点击“ Start Run "键,运行程序热循环程序文件( Protocol Tab )设置指南:点击 Edit 2、(编辑)或Create New (创建新程序)。反应板设置文件(Plate Tab )设置指南:选择本次实验所要使用的...
1.1 滴定分析法 滴定分析法先把未知浓度的CDNA 按一定比例稀释,同样把已知浓度CDNA 或参照也按比例稀释,在不同管内扩增得到合成DNA 的值,以起始CDNA 分子数N 0对合成DNA 分子数N 的对数LO G (N )作图,以此来估计不同样品中目标CDNA 的起始含量。1.2 动力学分析法 对于不同样品来说反映循环数n 决定了...