第二节 实时荧光定量PCR实时荧光定量PCR仪技术原理及应用一实时荧光定量PCR仪二实时荧光定量PCR的技术原理 1. 定量与常规的差别 2. 荧光扩增曲线 3. 荧光阈值和CT值 4. 熔解曲线 5. 标准曲线 6. 何为实时 7. SY
Report here the development of a sensitive, high-throughput real-time (RT)-PCR technique for estimating transgene copy number in GM rice. Use TaqMan quantitative RT-PCR and comparison to a novel rice endogenous reference gene coding for sucrose phosphate synthase (SPS) to determine the copy ...
MolecularBiologyoftheGene,6/E---Watsonetal.(2007)PartI:ChemistryandGeneticsPartII:MaintenanceoftheGenomePartIII:ExpressionoftheGenomePartIV:RegulationPartV:MethodsDNADNAPCRPCRDNADNAPCRPCRPolymerasechainreactionPolymerasechainreaction---AnimportanttechniquebasedonAnimportanttechniquebasedonDNAPolymeraseThepolymerase...
Gene Genomic structure / pseudogenes Regulation e.g. • 看家基因(Housekeeping genes):编码有基础细胞功能的蛋白 ß-actin multigene family; > 20 genes; 1 active locus : hormones of tyroid gland 质g-actin 20 pseudogenes multigene family; pseudogenes : stomach tumor GAPDH multigene family; 10-30...
Yotsuyanagi et al., “Regulation of Fas gene expression in HeLa cells as determined by modified RT-PCR”, Cell. Mol. Life Sci., vol. 54, 1998, p. 186-190.Yotsuyanagi et al., " Regulation of Fas gene expression in HeLa cells as determined by modified RT-PCR ", Cell. Mol. Life ...
Due to its importance in aquaculture and as a model for developmental studies, some gene expression studies have focused on the understanding of this process using quantitative real-time PCR (qRT-PCR) technique. Therefore, adequate reference genes for accurate normalization are required. Results The ...
(Gene Expression) 5. L. Wu, Y. Wu, B. Gathings, M. Wan, X. Li, W. Grizzle, Z. Liu, C. Lu, Z. Mao, x. Cao. Smad4 As A Transcription Corepressor for Estrogen Receptor a . JBC Papers In Press, Published on February7, 2003 as Manuscript M212332200. (Chromatin PPT.)6. L...
Continuous versus Cyclic Progesterone Exposure Differentially Regulates Hippocampal Gene Expression and Functional Profiles 伪subtype; Nr1h3), differed in direction of regulation by E2+CoP4 (down-regulation relative to OVX) and E2+CyP4 (up-regulation relative to OVX)... L Zhao,TE Morgan,Z Mao,....
亚型IgG ELISA Kit for Human Apelin0.625-40 ng/mL 纯化方法 affinity purified by Protein A 人水通道蛋白9(AQP9)ELISA试剂盒0.312-20 ng/mL 储存液 Preservative: 15mM Sodium Azid 具有下列特点: 1. 一站式,足够 50 次酶切-连接反应、50 次预扩反应和 1600 次 AFLP PCR(PCR 按 30 uL 体系计算),...
To deal with the increasing risk of pollutants from the aquaculture practice, wastewater treatment systems are considered to be an ideal strategy, for redu