实验方法原理DNA甲基化(DNA methylation)是最早发现的修饰途径之一,大量研究表明,DNA甲基化能引起染色质结构、DNA构象、DNA稳定性及DNA与蛋白质相互作用方式的改变,从而控制基因表达。 实验材料 样本DNA 试剂、试剂盒 NaOH对苯二酚重亚硫酸钠矿物油DNA Wizard cleanup kit (Promega) 或等同物异丙醇糖苷配糖基乙酸胺乙醇...
实验材料 样本 DNA试剂、试剂盒 NaOH对苯二酚重亚硫酸钠矿物油DNA Wizard cleanup kit (Promega) 或等同物异丙醇 糖苷配糖基乙酸胺乙醇10 X PCR 扩增缓冲液4dNTP 混合物Taq DNA 聚合酶仪器、耗材 水浴设备Vacuum manifold带控制管温度 2020-08-24 19:38 News WIKI 相关搜索 甲基...
DNA Wizard cleanup kit (Promega) or equivalent 80% isopropanol, room temperature Autoclaved distilled water, 60° to 70°C and room temperature Electrophoresis buffer (preferably TBE buffer; APPENDIX 2D) 10 mg/ml glycogen 10 M ammonium acetate 100% and 70% ethanol, ice cold 10× PCR amplificat...
The 5X Colorless GoTaq® Reaction Buffer is recommended for any applications where absorbance or fluorescence measurements of the PCR amplimer will be taken without prior cleanup. Both buffers are supplied at pH 8.5 and contain MgCl2 at a concentration of 7.5mM for a final concentration of 1.5...
1.一种用于进行免疫PCR以检测和/或定量一种或多种靶分析物,以及任选地检测和/后定量核酸的盒,所述盒包括:样品接收室;包含基质材料的室,所述基质材料作为滤器和/或DNA结合试剂起作用;温度控制的通道或室;和包含用于进行免疫PCR的试剂和/或缓冲液的多个室,其中:所述多个室包括含有捕获抗体的室,所述捕获抗体结合...
goldbio荧光素 联系方式 公司地址 苏州工业园区生物纳米园A4#216 联系电话 4000-520-616 / 18915418616 传真号码 0512-67156496 电子邮箱 info@ebiomall.com 公司网址 https://www.ebiomall.com Promega/Wizard® SV 96 PCR Clean-Up System/1kit/A9345 ...
产品特点KAPA Hyper Plus Kit, PCR-free (Illumina,8 rxns)二代测序文库制备 KAPA代理,KAPAKK8511,KAPA全国总代,KAPA KAPA Hyper Plus Kit, PCR-free (Illumina,8 rxns)二代测序文库制备 上海易汇生物科技有限公司,是一家集研发、销售为一体的技术生物企业,公司专注于生命科学和生物技术领域,专业提供分子生物学、...
Table 2 presents QS values of PCR templates derived from the cells of either agar colonies or clonal liquid cultures followed by one of the following DNA purification methods: LiCl-isopropanol DNA precipitation, PCR Cleanup, and gel electrophoresis followed by the extraction of DNA from agarose gel...
We have found on the basis of our comparative stain investigations, that the SEfiler Plus kit was less sensitive than the PowerPlex S5 and the MiniFiler kits. The MiniFiler and the PowerPlex S5 kit showed comparatively high sensitivity. Especially in analysing skin cell mixtures, the MiniFiler...
The resulting amplification products were extracted/purified using the Monarch PCR & DNA Cleanup kit (NEB), eluted in 30 µL of Nuclease-Free Water (Promega), and stored at −20 °C. Undiluted or a 10-fold dilution of the fusion PCR products were used as template DNA in either (i)...