PCR and its variants support a number of applications across the life sciences. Endpoint PCR is used to answer “yes/no” questions. For example: Did I successfully clone my gene fragment? Or: Is a given sequence present in a sample? The technique also can be used to prepare molecu...
PCR and antigen-based testing technologies The virus spread around the world in a few short months, and its variants continue to sweep the globe. The good news is that a robust testing program as part of an organization’s SARS-CoV-2 response can h...
It combines two powerful techniqueshybridization (ISH), which allows cellular localization of DNA and RNA sequences in cells and tissues, and the polymerase chain reaction (PCR), which allows reproducible amplification of rare nucleic acid sequences. The combined technique and its variants greatly ...
usamii AuFaeA and its variants were homologically modeled and optimized using the MODELLER 9.9 program (http://salilab.org/modeller/) with the crystal structure of AnFaeA (PDB code 1UWC) as a template. The B-factor Appl Microbiol Biotechnol (2015) 99:10047–10056 10049 values of the whole...
We designed an additional reaction to identify the pseudogene KIR3DP1 and its variants (KIR3DP1V), the latter characterized by the presence of an exon 2. The presence of KIR3DP1V on the chromosome 19 is associated with the absence of KIR2DL1 on the same haplotype5,24,25. Detection of ...
qPCR是一种选择性扩增和定量检测DNA或互补DNA（cDNA）区域的技术。位于感兴趣区域两侧的寡核苷酸引物用于...
Gives a broad overview of available PCR techniques and variants Focusing on the PCR technique, not restricted to a few limited applications associated with a minor discipline Chapters outlining appropriate quality control procedures are included Written by scientists with a broad knowledge of the field ...
variants, and generation of cDNA templates for cloning and sequencing. Since reverse transcription provides cDNA templates for PCR amplification and downstream experiments, it is one of the most critical steps for experimental success. Explore the following five steps to fast RT-...
(bla) genes are routinely carried out through tedious culture-based methods, whereas their definitive confirmation in Enterobacteriaceae and other bacteria is achieved largely by molecular methods.17 As the current subtypes and variants are continuously evolving and disseminating both geographically and ...
The situation of autosomal and X-chromosomal markers contrasts markedly with that of the Y chromosome, which exhibits in most of its length (Y-specific part) a haploid state and lack of recombination. Thus, Y chromosomes are transmitted by males to their male offspring and remain unaltered from...