ORFs cloned in this vector will be expressed in mammalian cellsas a tagged protein with the C-terminal Myc-DDK tags. (DDK is the same as FLAG? which is a registered trademark of Sigma Aldrich). Such clones are the best for detection and purification of the transgene using anti-Myc or an...
【目的】构建pCMV6-Entryp62真核表达质粒,并将其转染至人乳腺癌细胞株MCF-7中,通过抗生素筛选构建高表达p62的稳定细胞系。【方法】以293ET细胞cDNA为模板,设计引物扩增p62基因片段,双酶切扩增出的目的基因片段及pCMV6-Entry Vector空载体,将酶切后的片段进行连接,转化构建质粒,酶切及测序验证;将构建好的质粒转染入...
Mammalian vector for expressing myc- and FLAG®-tagged Cre to enable Cre-Lox recombination. Sequence Author: OriGene Open in SnapGene Try SnapGene for Free Download Plasmid|Download SnapGene Viewer Explore Over 2.7k Plasmids: Mammalian Expression Vectors | More Plasmid Sets No matches ...
【目的】构建pCMV6-Entry p62真核表达质粒,并将其转染至人乳腺癌细胞株MCF-7中,通过抗生素筛选构建高表达p62的稳定细胞系.【方法】以293ET细胞cDNA为模板,设计引物扩增p62基因片段,双酶切扩增出的目的基因片段及pCMV6-Entry Vector空载体,将酶切后的片段进行连接,转化构建质粒,酶切及测序验证;将构建好的质粒转染入...