Serve as the entry vector in the PrecisionShuttle system to transfer the ORF sequence into any destination vectors for other tagging options or other expression platforms. 多克隆位点示意图:免责声明:以上所展示的信息由企业自行提供,内容的真实性、准确性和合法性由发布企业负责,化工仪器网对此不承担任何保...
【目的】构建pCMV6-Entryp62真核表达质粒,并将其转染至人乳腺癌细胞株MCF-7中,通过抗生素筛选构建高表达p62的稳定细胞系。【方法】以293ET细胞cDNA为模板,设计引物扩增p62基因片段,双酶切扩增出的目的基因片段及pCMV6-Entry Vector空载体,将酶切后的片段进行连接,转化构建质粒,酶切及测序验证;将构建好的质粒转染入...
pCMV6-Entry-Cre Mammalian vector for expressing myc- and FLAG®-tagged Cre to enable Cre-Lox recombination. Sequence Author: OriGene Open in SnapGene Try SnapGene for Free Download Plasmid|Download SnapGene Viewer Explore Over 2.7k Plasmids: Mammalian Expression Vectors | More Plasmid Sets No matc...
【目的】构建pCMV6-Entry p62真核表达质粒,并将其转染至人乳腺癌细胞株MCF-7中,通过抗生素筛选构建高表达p62的稳定细胞系.【方法】以293ET细胞cDNA为模板,设计引物扩增p62基因片段,双酶切扩增出的目的基因片段及pCMV6-Entry Vector空载体,将酶切后的片段进行连接,转化构建质粒,酶切及测序验证;将构建好的质粒转染入...