Enhancer of zeste homolog 2(EZH2)is the catalytic subunit of polycomb repressive complex 2(PRC2).Dysregulation of EZH2 causes alteration of gene expression... X Wang,M Qi,X Zhu,... - 肿瘤学与转化医学:英文版 被引量: 0发表: 2023年 CLEC10A is A Prognostic Biomarker and is Correlated with...
GENE expressionCANCER cell proliferationBackground: Liver cancer is a prevalent and deadly form of cancer with high incidence and mortality rates. The PCMT1 protein has been linked to cell anti-apoptosis and tumor metastasis, but its significance in liver hepatocellular carcinoma (LIHC) remains ...
Expression system: Escherichia coli Tags: His tag C-Terminus, GST tag N-Terminus Background: This gene encodes a member of the type II class of protein carboxyl methyltransferase enzymes. The encoded enzyme plays a role in protein repair by recognizing and converting D-aspartyl and L-isoas...
we tried several siRNA and shRNA-mediated approaches to deplete PCMT1; however, although we achieved a dramatic reduction in the mRNA expression levels we were not able to significantly reduce PCMT1 protein levels (Supplementary Fig. 2). To circumvent...
Firefly luciferase was detected using Lumiscensor and t test analysis was done. Firefly luciferase expression was significantly decreased ( P <0.001) in comparison to the control. The low expression of firefly luciferase validates the method of target prediction that we used in this work by working...
Cancer is a commonly encountered disease both in Turkey and worldwide. Cellular gene expression profiles that drastically change during carcinogenesis can be regulated via several epigenetic mechanisms such as DNA methylation, non-coding RNAs (ncRNA), RNA interference (RNAi), ...