intuitive interventions, reactions from the MEP and isoprenoid biosynthesis pathways were not considered as the candidates for any form of intervention. Integer cuts were used to identify alternative optimal solutions (i.e., alternative genetic intervention choices) to achieve the minimum production yield...
This gene encodes glutamine synthetase, an essential enzyme in nitrogen metabolism that catalyzes the condensation of glutamate, a pivotal carbon skeleton, and free ammonia to form glutamine. This confirms our previous findings [11,12] when we reported an immediate and full shutdown of glnA ...