we thoroughly test our cell lines for freeze-thaw recovery. If frozen cells were received and not recovered in your lab under the exact, specified conditions (using recommended culture vessel, media, additional supplements, and atmospheric conditions), a live cell replacement is possible at a cost...
[15] MohamedIA,Krishnamoorthy NT,Nasrallah GK,et al.Theroleofcardiac myosinbindingprotein C3in hypertrophiccardiomyopathy-progressandnovelther- apeuticopportunities[J].J Cell Physiol,2017,232 (7):1650-1659. [16] 张智文,王婷,杨海涛.肥厚型心肌病家系携带 ACTN2,MYBPC3和 TNNI3基因突 变分析[J].临床...
Cells were cultured in 180 渭L media of RPMI1640, 12 K, or DMEM for 24 h. The purified compound with different concentrations was a...He QJ, Yang B, Lou YJ, et al. Contragestazol (DL111-IT) inhibits proliferation of human androgen-independent prostate cancer cell line PC3 in vitro ...
To determine if these integrins mediate adhesion of PC3 cells to collagen I and are responsible for the ephrin-A1-induced strengthening of cell adhesion we used integrin-blocking antibodies. Suspended PC3 cells were incubated in media containing antibodies against integrin β1-subunits44, integrin α...
In sham-irradiated cell, the cell culture media was removed for 12 min. a Detected distribution considering foci larger than 9 pixels. b Detected distribution considering foci larger than 30 pixels Full size image The two-sample Kolmogorov–Smirnov test comparing control and sham-irradiated nuclei ...
Membrane Interactome of a Recombinant Fragment of Human Surfactant Protein D Reveals GRP78 as a Novel Binding Partner in PC3, a Metastatic Prostate Cancer Cell Linedoi:10.3389/fimmu.2020.600660Gargi ThakurGajanan SatheIndra KunduTaruna MadanFrontiers Media SA...
Cells were maintained in RPMI-1640 media with L-glutamine supplemented with fetal bovine serum (50 ml fetal bovine serum/500 ml media) at 37 °C 5% CO2. PC3 and LNCaP cell lines were validated by Idexx Radil (Columbia, MO, USA) on 24 December 2012. Sulforaphane was purchased from LKT...
treated cells. In each well of the incubated cells, culture media were removed and a neutral red medium (100 µl) was added and then incubated for 2 h to allow for vital dye incorporation into living cells. The neutral red media were decanted, and Dulbecco’s PBS buffer (150 µl) ...
The hiPSCs were generated from peripheral blood mononuclear cells (PBMCs) collected from age-matched noncarrier or D389V carrier human subjects as described previously [20,35]. All hiPSCs were maintained and expanded in mTeSR™1 media. To form 3D aggregates, cells were placed in agarose molds...