(4) it is easy to divide rapidly and can be continuously subcultured to provide large numbers of cells in a short period of time. As regards neuronal cell lines, they can be induced to proliferate or differentiate by treating with serum growth factors or a variety of drugs. This ability ...
都是single cell。用这种方法subculture的时候,建议用10cm dish养。flask的话吹打起来不方便,肯定不能吹打均匀。 我也没有每次都换dish,一般是一礼拜换一次,但细胞状态不好的时候,每次subculture时都换新dish。 细胞刚养的时候一定要多冻几管,万一不好也有back-up可以重新再来。还有一个办法就是常换medium、营养...
Aim:To study the cell division, neurite extension, migration of neurons differentiated by NGF in subcultured PC12 cells.Methods: The Giemsa staining, methyl green-pyronin staining and proliferating cell nuclear antigen immunohistochemical assay were used to study the subcultured PC12 cells.Results: ...
PC12细胞的培养整理总结 一、PC12细胞的折光性本来就比较好,刚传代以及新分裂的细胞都是亮的,至于你说的贴壁的细胞,不知道是否是圆的,在牛血清的刺激下,特别是国产的,会使pc12细胞发生定向的分化,长突起,贴壁就比较牢。 PC12分为未分化的和分化的.前者是球状,成团生长;后者形成很多丝状体;而丁香园里有人说...
To assess cellular ROS generation, the measurement of 2′,7′-dichlorodihydrofluorescein diacetate (H2DCF-DA; Molecular Probes) fluorescence intensity was used. This originally non-fluorescent reagent diffuses passively through the cellular membrane into the cell, where acetate groups are cleaved by in...
Cell culture and transfection PC12 cells were maintained and subcultured as previously described in detail [24]. The cells were plated onto collagen-coated (Biomedical Technologies Inc., Stoughton, MA, USA) culture dishes (NUNC, Roskilde, Denmark) and were kept in OptiMEM (InVitrogen, Vienna, ...
To confirm that PLD1 is also activated by PMA treatment, we created an inducible PC12 Tet-Off cell line, which overexpressed PLD1 in the absence of tetracycline. On checking the PMA-induced PLD1 activity in these PC12 cells, we found that the activity of PLD1 was also enhanced by the ...
【Method】 :First culturing PC 12 cells, then digest and subculture it when adherent cells is about 70%.PC 12 cells which grow well is made into cell suspension that is inoculated into 96-hole culture plate, each hole against 100µL. Place it in the incubator for 24 hours. Separate it...
(100 units/mL penicillin, 100 μg/mL streptomycin; Welgene). The medium was replaced every 2 days, and the subculture was repeated for four days. The cells were incubated in a 5% CO2humidified incubator at 37 °C and were seeded at 1 × 106cells/well in 96-well culture plate (pre-...
www.nature.com/scientificreports OPEN Optimisation of a PC12 cell‑based in vitro stroke model for screening neuroprotective agents PinFen Chua & William K. Lim* Stroke causes death and disability globally but no neuroprotectant is approved for post-stroke neuronal injury. ...