In addition, MSCs significantly decreased apoptosis of PBMCs in co-culture with HeLa at 48 h. the p-value was 0.0022. Real-time PCR showed that the expression of TGF-尾 in PBMCs/MSCs/HeLa co-culture increased after 24 h, with a p-value of 0.006. C...
#B细胞 前段时间,有读者私信我想让我出一期关于B淋巴细胞体外培养的内容。相比于T细胞,B细胞体外培养的难度较大,并且与B细胞体外培养相关的文献也相对较少 。笔者在此对几篇B细胞体外扩增和培养的文献进行了总结,为大家提供了几种体外培养B细胞...
5. Xu, H., et al.,Influence of various medium environment to in vitro human T cell culture.In Vitro Cellular & Developmental Biology. Animal, 2018.54(8): p. 559-566. 6. Sato, K., et al.,Impact of culture medium on the expa...
CD4+ T cells (1 x105) and allogeneic DCs (1 x 104) were co-cultured with or without dosetitrations of nivolumab added at the initiation of the assay. After 5 days, IFN secretion in culture supernatants was analyzed by ELISA, and cells were labeled with 3H-thymidine for an additional 18...
In this study we show that, whilst hESC-EC do not respond to LPS (1μg/ml) to release CXCL8 when treated in mono-culture, they mediate a synergy when co-cultured with peripheral blood mononuclear cells (PBMCs). No synergy was found in co-cultures stimulated with TNFα (10ng/ml) ...
are then separated from the untouched desired PBMCs by using an EasySep™ magnet and simply pouring or pipetting the desired cells into a new tube. Following magnetic cell isolation, the desired PBMCs are ready for downstream applications such as flow cytometry, culture, or DNA/RNA extraction....
57-64.5. Caeser, R., et al.,Genetic manipulation and immortalized culture of ex vivo primary human germinal center B cells. Nature Protocols, 2021. 16(5): p. 2499-2519.6. Kuo, T.C., et al., Repression of BCL-6 is required for the formation of human memory B cells in vitro. ...
使用CytoTune®-iPS 重编程试剂盒重编程外周血单核细胞 (PBMC),CytoTune-iPS 仙台病毒重编程系统利用基于在感受态仙台病毒中进行复制的载体,安全高效地递送和表达将体细胞重编程为iPSC所必需的关键遗传因子。
Protocol for HIV-1 Isolation by PBMC Co-Culture (January …:通过外周血单个核细胞共培养的HIV-1分离协议(一月… 热度: Cleavage, Deprotection, and Isolation of Peptides after Fmoc Synthesis 热度: Isolation and Culture of Intestinal Epithelial Cells 热度: 相关推荐 外周血单个核细胞分离 密度梯度...
The impact of M-CSF and RANKL in the common OC co-culture was assessed for OB, FB and OK via MTT assay via DAPI control. The multinuclearity and function of OC were evaluated by light microscopy, DAPI staining, resorption assay and FACS analysis. The PBMC showed the highest differentiation...