& Jackson, S. P. Regulation of DNA-damage responses and cell-cycle progression by the chromatin remodelling factor CHD4. EMBO J. 29, 3130–3139 (2010). Article PubMed PubMed Central CAS Google Scholar Gong, L. et al. p53 isoform D113p53/D133p53 promotes DNA double-strand break repair...
Goat anti-rabbit IgG secondary antibody (JACKSON, catalog: 111–035-003) was incubated and DAB solution (Sigma, catalog: D8001) was used for color development. All images were obtained using an Open-field slice scanner (NanoZoomer S210). Osteoclastogenesis assays The osteoclastogenesis assays were...
In brief, after 100 ml plasma was injected, lipoproteins were run at 0.5 ml min À 1 in a buffer containing 0.15 M NaCl, 0.01 M Na2HPO4, 0.1 mM EDTA, pH 7.5, and separated on a Superose 6 10/300 GL column (GE Healthcare) by using BioLogic DuoFlow QuadTec 10 System (Bio-Rad,...
The CXCR4 antibody synergizes with CLL standard of care agents To determine if the CXCR4 antibody could offer additional benefit to available therapies, the m15-IgG1 antibody was evaluated in combination with SOC agents currently used in the treatment of CLL patients in the clinic. The percent ...
(Cell Signaling Technology), a-SNAI1 goat pAB (R&D Systems), a-Myosin Heavy Chain mouse mAb (monoclonal antibody) myosin heavy chain (a-MF20, R&D), MEF2C rabbit mAb (CST); and secondary antibodies: HRP (horseradish peroxidase) anti-rabbit (CST, 7074) or HRP anti-mouse (GE Healthcare...
For TAMs isolation, the single-cell suspensions were centrifuged at 500 × g for 20 min, with 1 mL of the cell suspension on the top, 5 mL of 45% Percoll (GE Healthcare, 17-0891-09) in the middle and 5 mL of 60% Percoll at the bottom of a 15-mL tube. Mononuclear cells ...
Autosomal dominant polycystic kidney disease (ADPKD) is caused by mutations in PKD1 or PKD2 which encodes polycystin-1 (PC1) and polycystin-2, respectively. PC1 was previously shown to slow cell proliferation and inhibit apoptosis but the underlying mechanisms remain elusive or controversial. Here...
Sirtuins are NAD+-dependent deacetylases that facilitate cellular stress response. They include SirT6, which protects genome stability and regulates metabolic homeostasis through gene silencing, and whose loss induces an accelerated aging phenotype direc
The animal protocol was approved by the Institutional Animal Care and Use Committee of the University of Arkansas for Medical Sciences (UAMS). Male C57BL/6 mice were obtained from The Jackson Laboratories (Bar Harbor, ME, USA); mice between 8 and 12 weeks of age were used for experiments....
Vaccines based on the spike protein of SARS-CoV-2 are a cornerstone of the public health response to COVID-19. The emergence of hypermutated, increasingly transmissible variants of concern (VOCs) threaten this strategy. Omicron (B.1.1.529), the fifth VOC