Patch Whole Patch Inside-Out Patch Outside-Out The patch technique is based on the electrical isolation of a small patch of membrane from the rest of the cell. To achieve this isolation, the patch pipette is placed against the cell membrane, and a slight suction or negative pressure is ...
The technique depends on the electrical and physical isolation of one compartment, the pipette lumen, from another, the surrounding bath. The isolation of the lumen of the pipette is the essential feature of the patch clamp technique; failures in isolation introduce artifacts, always excess ...
Pipette driftJoining an established electrophysiology laboratory typically means that the micropipette fabrication process has already been perfected allowing newcomers to be trained without a complete understandidoi:10.1007/978-1-4939-3274-0_5Nicholas Graziane...
Elevating internal Cl via the patch pipette invariably slowed endocytosis, even in terminals dialyzed with increased Ca 2+ buffer. These results suggest a ... C Hull,HV Gersdorff - 《Neuron》 被引量: 100发表: 2004年 Fast and High Quality Overlap Repair for Patch-Based Texture Synthesis Patch...
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In whole-cell configuration, the pipette first makes a seal on the plasma membrane. Apulseof suction applied through the pipette ruptures the membrane and creates direct continuity between the pipette solution and the interior of the cell. In whole-cell configuration, the entire plasma membrane is...
transmembrane proteins (e.g.,membrane receptors, ion channels). Such proteins will not migrate into or out of the membrane under the patch pipette. Therefore, only diffusible cytosolic second messengers (•) or membrane components under the patch pipette can influence the electrical activity ...
BLMs(bilayer lipid membranes)nucleic acid biosensor supported by patch-clamp pipette electrode was used to detect staphylococcus enterotoxins B(SEB)gene.De... L Nan,ZX Gao - 《Chinese Journal of Analytical Chemistry》 被引量: 3发表: 2007年 ...
BLMs(bilayer lipid membranes)nucleic acid biosensor supported by patch-clamp pipette electrode was used to detect staphylococcus enterotoxins B(SEB)gene.De... L Nan,ZX Gao - 《Chinese Journal of Analytical Chemistry》 被引量: 3发表: 2007年 Mechanical Behavior of GFRP Laminated Composite Pipe Sub...