Patch-clampPipette detectionAutopatchingEnergy minimizationLabel-freeSegmentation of objects with known geometries in an image is a wide research area. In this paper we show an energy minimization model to detect the tip of glass pipettes in microscopy images. The described model fits two rectangles ...
Patch Clamp Techniques Inpatch clampmethods, a glass micropipette is used to make tight contact with a tiny area, or patch, ofneuronal membrane. After applying a small amount of suction to the back of the pipette, the seal between pipette and membrane becomes so tight that ions can no longe...
Patch Pipettes The patch clamp technique is used in electrophysiological research to study the electrical activity of neurons at the cellular level. The technique requires using a blunt pipette with a 3-4mm short taper and a 1-3μm tip to isolate a patch of membrane. In general, patch ...
To achieve a successful recording, the patch-clamp pipette must have a clean tip to form a high-resistance (≥1 GΩ) junction (or gigaseal) with the cell membrane (Fig. 1a). In his Nobel Prize lecture, Edwin Neher remarked that "a gigaseal could be obtained reproducibly...
Patch-clamp recording is a gold-standard technique for the measurement of membrane voltage and current fluctuations in electrically active cells. From the advent of the technique in the 1970s, it has been widely accepted that using a new, un-contaminated pipette to patch-clamp every cell is a...
The electrode within the pipette is connected to an amplifier that can amplifies current and voltage fluctuations that are the result of the movement of ions through channels in the plasma membrane. With the amplifier, scientists can clamp or artificially set the membrane potential at specific voltag...
Axopatch Patch Clamp Amplifier is the premier microelectrode amplifier for ultra-low noise patch clamp recordings. It helps in current-clamp recordings, field potential recordings in ion channel and high-speed current clamp recording within the same head
This article gives an introduction to the patch-clamp technique and how it is used to study the physiology of ion channels for neuroscience and other life-science fields.
automatic or manual adjustment of the pipette offset in a range of +/- 200 mV automatic or manual Leak subtraction possibility of full manual control of the amplifier improved headstage design to reduce noise levels additional amplification range for large currents (up to 2 µA) variable, high...
The Patch-clamp technique is a versatile electrophysiological tool for understanding ion channel behavior and neurons, muscle fibers, cardiomyocytes, and oocytes overexpressing.