Cultured cell lines are the workhorse of cancer research, but the extent to which they recapitulate the heterogeneity observed among malignant cells in tumors is unclear. Here we used multiplexed single-cell RNA-seq to profile 198 cancer cell lines from 22 cancer types. We identified 12 expression...
single-cell RNA sequencing.Objective: Varied expression of drug-metabolizing enzymes (DME) genes dictates the intensity and duration of drug response in cancer treatment. This study aimed to investigate the transcriptional profile of DMEs in tumor microenvironment (TME) at single-cell level and their...
Scientists depicts pan-cancer single-cell landscape of tumor-infiltrating T cells A joint study by two groups of researchers at Peking University revealed for the first time the “pan-cancer single-cell landscape of tumor-infiltrating T cells”. The study analyzed tumor-infiltrat...
内容提示: RESEARCH ARTICLE SUMMARY◥CANCER IMMUNOLOGYPan-cancer single cell landscapeof tumor-infiltrating T cellsLiangtao Zheng†, Shishang Qin†, Wen Si†, Anqiang Wang, Baocai Xing, Ranran Gao, Xianwen Ren,Li Wang, Xiaojiang Wu, Ji Zhang, Nan Wu, Ning Zhang, Hong Zheng, Hanqiang ...
Pan-Cancer Single-Nucleus Total RNA Sequencing Using snHH-Seq 说是说snHH-seq,实际上就是M20平台 总体来说,这个数据是用的pan-cancer的forzen sample,虽然不如成年FFPE降解的多,但是一般用普通单细胞的话,还是会碰到一些RNA降解的问题。所以用细胞核RNA的话比较合适。
To establish a spatial single-cell landscape in pan-cancer, we acquired scRNA-seq data from 69 samples of 56 patients diagnosed with one of the six prevalent cancer types, along with ST data from 22 tissue slices of 22 patients (Fig.1a and b; Table S1and S2). Among them, the ST data...
Landscape of infiltrating t cells in liver cancer revealed by single-cell sequencing. Cell 169, 1342–1356 (2017). CAS PubMed Google Scholar Crinier, A. et al. High-dimensional single-cell analysis identifies organ-specific signatures and conserved NK cell subsets in humans and mice. Immunity...
DNA methylation instability TCGA pan-cancer cohort pediatric cancer Introduction The cell epigenome provides a fundamental infrastructure to coordinate spatiotemporal gene expression and cell-type-specific patterns (Shen and Laird, 2013). This organization ranges from directly decorating DNA molecules and his...
Figure 1. Overview of the cohorts and proteomic landscape of therapeutic targets (A) Number of tumor and normal tissue samples for 10 cancer cohorts and number of total identified features for each omics type. (B) Number of genes present in each of five target tiers. Overlapped genes were as...
Single-cell analysis was performed (https://www.home-for-researchers.com/) to evaluate differences in disulfidptosis-related gene expression at the single-cell level. We employed Seurat, an R package, to execute quality control on pan-cancer data. Principal component analysis was utilized to de...