Subsequently, stable β-catenin facilitated the formation of the Pol II–DSIF–PAF1C complex, suggesting that stable β-catenin induces cancer stemness by stimulating active Pol II complex through NELF and PAF1C. Furthermore, NELF or PAF1C inhibition recapitulated the changes in cancer stemness...
1C is capable of assembling in the absence of the Cdc73 C-domain; however, given the proximity of the C-domain to sequences required for Paf1C assembly, we cannot discount the possibility that the C-domain has a more subtle effect on Paf1C stability or the conformation of the complex....
Paf1C comprises the five subunits Paf1, Leo1, Ctr9, Cdc73 and Rtf1, and binds to the RNA polymerase II (Pol II) transcription elongation complex (EC). Here we report the reconstitution of Paf1C from Saccharomyces cerevisiae, and a structural analysis of Paf1C bound to a Pol II EC ...
Top: SPT6 facilitates the recruitment of PAF1 complex and removal of NELF complex. This leads to the release of RNA Pol II into gene bodies with high processivity. Note that SPT6 may indirectly regulate this NELF-PAF1C exchange, mediated through SPT6-interacting factors. Other factors such ...
In pancreatic ductal adenocarcinoma (PDAC), endogenous MYC is required for S-phase progression and escape from immune surveillance. Here we show that MYC in PDAC cells is needed for the recruitment of the PAF1c transcription elongation complex to RNA polymerase and that depletion of CTR9, a ...
PD2 (Pancreatic Differentiation 2) or RNA Polymerase II-Associated Factor 1 (Paf1) is a core component of human PAF1 complex (PAF1C), which regulates transcription elongation and mRNA processing. Human PAF1C consists of 5 members: PAF1, LEO1, CDC73, CTR9 and SKI8. Recently, PAF1 has...
In this dissertation, we further characterize the molecular mechanisms regulating two factors that function during RNAPII elongation.;The Paf1 complex (Paf1C) is involved in a variety of processes during elongation, however it is unknown if the Paf1C can directly affect the elongation activity of...
The Paf1 Complex Has Functions Independent of Actively Transcribing RNA Polymerase II. Mol Cell 2004; 14:447-56.5. Penheiter KL, Washburn TM, Porter SE, Hoffman MG, Jaehning JA. A posttranscriptional role for the yeast Paf1-RNA polymerase II complex is revealed by identification of primary ...
Leo1 is a component of the evolutionarily conserved RNA polymerase II-associated factor 1 complex (PAF1C), which is a positive regulator of transcription elongation. The dalknu6 mutant embryos specified motor neurons and OPCs normally, and at the appropriate time, but OPCs subsequently failed to...
Paf1 (Polymerase-associated factor 1) complex (Paf1C) is evolutionarily conserved from yeast to humans, and facilitates transcription elongation as well as co-transcriptional histone covalent modifications and mRNA 3 '-end processing. Thus, Paf1C is a key player in regulation of eukaryotic gene ...