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endothelial cells, fixed tumor cryosections prepared from tumor bearing mice treated with PTX drug alone and Bcl-2 siRNA formulated liposomes were immunostained with tumor endothelial cell marker VE-cadherin and TUNEL following manufacturer’s protocol (Clontech Laboratories, Mountain View, CA, USA). ...
Plasmid transfection was performed using Lipofectamine® 2000 (Invitrogen, Waltham, MA, USA) according to the manufacturer’s protocol. Briefly, a 2 μg of plasmid in optiMEM media was mixed with 4 μl of Lipofectamine® 2000 and incubated for 15 min at room temperature. The ...
Plasmids were transfected in ovarian cancer cells using Lipo3000-based Transfection Reagent (System Invetrogen Inc., Palo Alto, CA, USA), according to the manufacturer’s instructions. Western Blotting Whole-cell and cytoplasmic proteins were extracted using a ProteoJET Kit (Fermentas, USA). ...
(1 µM paclitaxel equivalent) followed by US-exposure to the respective groups. The cells were further incubated for 48 hours. Following the incubation, apoptosis assay was performed according to the manufacturer’s protocol. FACS data was acquired and analyzed using BD FACS Aria (BD ...