The HiFi difference — not being CLR This week Illumina gave an update on their synthetic long-read approach previously named “Infinity reads,” now rebranded as Complete Long Reads (CLR). This acronym choice is of course quite ironic since CLR – Continuous Long Reads— was the first Pac...
September 30, 2022 | HiFi difference The HiFi difference — not being CLR This week Illumina gave an update on their synthetic long-read approach previously named “Infinity reads,” now rebranded as Complete Long Reads (CLR). This acronym choice is of course quite ironic… Read more Sep...
PacBio HiFi long-read DNA sequencing, so-called circular consensus sequencing (CCS), greatly improves the accuracy of third generation long-read sequencing compared to original continuous long-read (CLR) sequencing [1]. This method requires high molecular weight, high quality DNA as input material...
This discrepancy aligns with the accuracy gradient across HiFi, CLR, and ONT sequencing45,46. Moreover, 98.7% of the conserved single-copy genes were assembled in the ISO1-1/2 assemblies, while the proportions for previous assemblies were generally lower (median: 97.2%). The reference genome ...
(generated in the less accurate continuous long-read mode or CLR mode) to 63 (HiFi mode), while the three ONT-based assemblies exhibited lower values (28–38). This discrepancy aligns with the accuracy gradient across HiFi, CLR, and ONT sequencing45,46. Moreover, 98.7% of the conserved ...
Accurate assembly of segmental duplications, satellites, and allelic variants from HiFi readsHifiasm A fast, haplotype-resolved genome assemblerPeregrine A fast genome assembler for accurate long readsFor assemblies of continuous long reads (CLR) use HGAP4 analysis applicationSMRT...
Accurate assembly of segmental duplications, satellites, and allelic variants from HiFi readsHifiasm A fast, haplotype-resolved genome assemblerPeregrine A fast genome assembler for accurate long readsFor assemblies of continuous long reads (CLR) use HGAP4 analysis applicationSMRT compatible analysis product...