of Tau neuropathology at molecular level, we used cDNA arrays representing 8832 genes to determine the mRNA expression profile in transgenic mice expressing the most common frontotemporal dementia and parkinsonism linked to chromosome 17 (FTDP-17) Tau mutation (P301L) (Nat. Genet. (2000) 402). ...
［作者简介］马登磊（１９８８ ），男，博士研究生，研究方向：神经药理学㊂Ｅ⁃ｍａｉｌ：ｍａ＿ｄｅｎｇｌｅｉ＠１２６．ｃｏｍ ［通信作者］李林，教授，研究方向：神经药理学㊂Ｅ⁃ｍａｉｌ：ｌｉｎｌｉｘｗ＠１２６．ｃｏｍ 研究进展Ｐ３０１Ｌ突变ｔａｕ蛋白转基因动物模型及其应用 马登磊，张如意，李㊀林∗...
证明,外源性人tau基因已整合到小鼠基因组内,在mRNA和蛋白水平都有表 达,免疫组织化学显示P301L突变的tau转基因小鼠具备了部分tau病理改变。 8只阳性转基因小鼠的Morris水迷宫实验数据与8只阴性对照鼠的实验数据比, 虽无显著统计学差异,但从趋势看,阳性转基因小鼠可能存在空间学习能力的障 ...
要: 微管相关蛋白tau在阿尔茨海默病 (Alzheimer’s Disease, AD) 等多个tau蛋白病 (tauopathies) 的发病机制中发挥重要的作用, 并且得到了越来越多的关注。在tau蛋白病的研究中, 理想的tau蛋白病变模型对于发病机制和药物治疗的研究具有重要的意义。目前国内外学者已经建立了多个tau蛋白转基因动物模型, 其中过表达P...
Kobayashi T, Mori H, Okuma Y, Dickson DW, Cookson N, Tsuboi Y, Motoi Y, Tanaka R, Miyashita N, Anno M, Narabayashi H, Mizuno Y (2002) Contrasting genotypes of the tau gene in two phenotypically distinct patients with P301L mutation of frontotemporal dementia and parkinsonism linked to ...
Here we show that these mice have abnormal tau filaments not only in neurons, but also in oligodendrocytes and astrocytes. Similar results were detected in another transgenic line (JNPL2+3+) that expresses the longest human tau isoform with the P301L mutation. The ultrastructure of the tau ...
The mice were generated with a tau cDNA containing exon 1, 4, 5, 7, 9, 10, 11-13, intron 13 and exon 14, driven by the mouse prion promoter (MoPrP) and containing the most common FTDP-17 mutation, P301L. The transgenic construct was generated by ligating the P301L tau cDNA ...
Intracellular accumulation of abnormally phosphorylated tau in different types of neurons is a pathological characteristic of Alzheimer’s disease (AD). While tau modification and associated neuronal loss and hypometabolism start in the entorhinal cortex
Presence of Reactive Microglia and Neuroinflammatory Mediators in a Case of Frontotemporal Dementia with P301S Mutation Recent findings, showing the presence of an inflammatory process in the brain of transgenic mice expressing P301S mutated human tau protein, indicate that ... A Bellucci,O Bugiani...
The disease is caused by a P301L mutation in microtubule-associated protein tau (MAPT). Induced pluripotent stem cells (iPSCs) were established by electroporation with episomal plasmids containing hOCT4 , hSOX2 , hKLF2 , hL-MYC , hLIN-28 and shP53 . iPSCs were free of genomically ...