Briefly, total blood was diluted with PBS (1:1), and 6 ml of diluted blood was gently mixed with 3 ml of LymphoprepTM density gradient solution (Serumwerk Bernburg AG, Oslo, Norway), and centrifuged at 800×g for 30 min at room temperature. Cells were collected from the interphase layer...
A 5-mL sample of anti-coagulant peripheral blood was taken from each participant. Genomic DNA was extracted from whole blood samples using a Qiagen kit (Qiagen, Hilden, Germany) according to the manufacturer’s recommendations and stored at –80 °C until further use. All SNPs were genotyped...
whole blood platelet aggregationObjective. Several platelet function test systems exist for the evaluation of the platelet inhibitory effect in patients on P2Y12 inhibitors and/or acetylsalicylic acid (ASA, aspirin) thera...doi:http://dx.doi.org/10.3109/00365513.2014.913305Jan-Christopher Krüger...
They function as the predominant receptor in microglial process chemotaxis toward injury (Haynes et al., 2006) and can heal the compromised blood-brain barrier by this chemotactic mechanism (Lou et al., 2016). Moreover, they mediate various microglial-to-neuronal physical interactions (Eyo et al...
The instrument aspirates a blood sample under constant vacuum from the sample reservoir through a capillary and a microscopic aperture (147 μm) cut into the membrane, which leads to high shear–induced platelet plug formation (72). For the detection of the effects of ADP antagonists, the ...
demonstrated that pre-operative exposure to clopidogrel was associated with an increased risk of death (RR: 1.3; CI: 1.02–1.67), re-operation for bleeding (RR: 1.88; CI: 1.37–2.58) and need for packed red blood cells (RR: 1.23; CI: 1.10–1.37) [6]. ACCOAST is the only other ...
Although chemotherapy has a therapeutic scope and considerable penetration in the blood-brain barrier, temozolomide resistance has been broadly described in GBM patients [4,5]. The analysis of the TME, where neoplastic and non-neoplastic cells interact, has been shown to be fundamental in ...
Journal of Clinical Medicine Article P2Y12 Inhibitor Pre-Treatment in Non-ST-Elevation Acute Coronary Syndrome: A Decision-Analytic Model James Gunton 1, Trent Hartshorne 1, Jeremy Langrish 1,2, Anthony Chuang 1 and Derek Chew 1,* 1 Cardiology Department, Flinders University/Southern Adelaide ...