StrokeClopidogrel is the most-widely used platelet P2Y12-inhibitor for secondary-prevention of ischemic stroke. Platelet P2Y12 reactivity before and after inhibitors can be measured with blood sampling by commercialized system. We aimed to evaluate (1) whether high-on-clopidogrel platelet P2Y12 ...
10-180 PRU [P2Y12 Reaction Units] - Decreased platelet reactivity to P2Y12 inhibitor. 2. VerifyNow-Aspirin Assay: ≥ 550 Aspirin Reaction Units [ARU] - No evidence of platelet dysfunction due to Aspirin. < 550 Aspirin Reaction Units [ARU] - Platelet dysfunction consistent with Aspirin - detec...
Platelet aggregation on ADP agonist and whole blood count (CBC) were assessed at baseline and repeated 1 h and 3 h after the loading dose (to test onset and peak of drug action), at day 4 (after reaching steady-state), 12 and 24 h after discontinuation of the antiplatelet drug (to ev...
For microfluidic studies, whole blood (1 ml) was collected in a syringe containing the thrombin inhibitor H-D-Phe-Pro-Arg-chloromethylketone (PPACK) to maintain extracellular cation concentrations and thus preserve integrin function. For light transmission aggregometry (LTA) studies, citrated blood ...
P2Y12 receptor inhibitor plus aspirin versus aspirin treated within 24 h of acute noncardioembolic ischemic stroke or TIA: meta-analysis J Formos Med Assoc (2021), 10.1016/j.jfma.2021.08.006 Google Scholar 11 G.A. Medranda, C. Zhang, G. Doros, C. Yerasi, B.C. Case, W.S. Weintraub,...
Blood was collected from the post-orbital venous plexus vein at different times after the ConA injection, and the serum was separated by centrifugation at 4,000×g at 4 °C for 10 min. Serum levels of alanine aminotransferase test (ALT) and aspartate aminotransferase test (AST) were measured...
The protein of the collected mouse brain tissues was extracted using cold lysis buffer containing protease inhibitor. The protein concentrations were determined by the bicinchoninic acid (BCA) method (Beyotime Biotechnology, Shanghai, China). Equal amounts of protein from different samples were separated...
This open-label randomized trial compares the effect of a genotype-guided oral P2Y12 inhibitor selection strategy vs conventional clopidogrel prescribing
Advantages of the VerifyNow assay include full automation, use of whole blood, good reproducibility, bedside use, and small blood volume requirement. The most important disadvantage is that the test kits are expensive. The normal value (without P2Y12 receptor blockers) is >235 P2Y12 reaction unit...
Glucuronidation converts clopidogrel to a strong time-dependent inhibitor of CYP2C8: a phase II metabolite as a perpetrator of drug–drug interactions. Clin Pharmacol Ther. 2014;96:498–507. CAS PubMed Google Scholar US Food and Drug Administration and Center for Drug Evaluation and Research....