A peroxidase activity with two substrates can occur on the surface of GNZs with a ping-pong mechanism similar to natural enzyme. This type of reaction on GNZs conforms a similar ping-pong mechanism in two steps similar to natural enzymes [28]. In the first step, H2O2 as the first ...
The largest subunit of FP which contains FMN and the iron–sulfur cluster N-3 has been identified to contain a single NADH-binding site in the mammalian and bacterial Complex I [95–98]. On the other hand, since FP is capable of transhydrogenase activity it was suggested that FP conta...
The influence of nineteen flavonoids on cow's milk xanthine oxidase (xanthine: oxygen oxidoreductase, EC 1.2.3.2) was investigated. The enzyme activity was estimated by measuring the increase in absorbance at 290 nm due to uricate format... Masayoshi,Iio,Ayako,... - 《Agricultural & Biological...
In general, the new inhibitors can be classified into two categories: those blocking a single virulence factor, such as adhesin or toxin, and those with a global activity to block major bacterial processes such as quorum sensing, the two-components system, secretion systems or post-translational ...
of the species name [35]. Once a protein is experimentally characterized, a name is then assigned based on its enzymatic function with the first letter capitalized (Table1). The proteins without known activity are represented by AA3_2, while paralogs are denoted by a single uppercase letter....
The EF-hand motif, for example, was proposed to regulate the NDH-2 activity in a calcium dependent manner16. These motifs may constitute sites for regulation of enzyme activity by acting on the residues that stabilize/protonate FAD in different oxidation states. Also, the distribution of NDH-...
A recent study has demonstrated that CLIC1,2 and CLIC4 have glutaredoxin-like activity, with the conserved cysteine at the N-terminal GST fold acting as the key catalytic residue29. Here we demonstrate that CLIC3 is also a GSH-dependent oxidoreductase and that cysteine 22 is the necessary ...
Cytochromes were similarly tested for selenate and selenite reductase activity by incubation with the relevant substrate but no SerABC. Optical Redox Titrations Optical spectra of the samples were measured on a Hitachi U-3310 spectrophotometer between 350 and 700 nm. Protein samples were added to a...
(containing two WW domains) and variable-length C termini where sequences necessary for oxidoreductase activity are located. A nuclear localization signal is also located between the WW domains. The most abundant spliced form encodes a protein that has been named FORII, WWOX, and WOXl [41,95,...
We, therefore, tested the effect of Rev on ubiquitinated Tat as described earlier30. Rev potently reduced the ubiquitinated forms of Tat (Fig. 2c), which may explain the reduced functional activity of Tat shown earlier. The above results suggest that Rev induces proteasomal degradation of Tat ...