Depending on the same parameters, XDH can also produce these ROS at the FAD site by acting as a NADH oxidase. This activity is retained when the molybdenum or sulfur atoms are missing in the molybdopterin cofactor, as well as when XOR is inhibited by competing or non-competing drugs, ...
activity. A third strictly conserved pair, G301D302, forming the third conserved motif, is observed close to O3* of FAD (Figs 1Band2). The backbone of G301establishes a hydrogen bond (2.7 Å) with the side chain of a residue located after the proposed quinone binding site motif (A31...
Journals & Books Help Search My accountSign in View PDF Download full issue Search ScienceDirect FEBS Letters Volume 581, Issue 29, 11 December 2007, Pages 5645-5648EXAFS reveals a structural zinc binding site in the bovine NADH-Q oxidoreductase Ed...
One unit of enzyme activity was defined as the reduction of 2 μmol·min−1 of metronidazole (corresponding to the decarboxylation of 1 μmol·min−1 of pyruvate on the assumption that the bleaching of the chromophore is a one-electron process) [55]. The reductive carboxylation ...
The gray area represents the membrane and curved arrows schematize NADH:quinone oxidoreductase activity; (B) Cartoon representation of a zoomed view of the FAD region and co-crystallized ubiquinone and NADH of the NDH-2 from S. cerevisiae (PDB:4G734). The atoms of the FAD group are ordered...
Xanthine oxidoreductase (XOR) and its products contribute to the development of chronic inflammation and oxidative stress. Excessive XOR activity is believed to promote inflammatory responses and atherosclerotic plaque formation, which are major cardiova