Toward this end, and based on our previous work demonstrating the metabolic differences that exist between PaCSCs and non-PaCSCs, we describe herein a long-term and sustained cell culture system enriched in PaCSCs based on forced oxidative phosphorylation (OXPHOS), defined by electron transport ...
Reprogramming hematopoietic stem cell metabolism in lung cancer: glycolysis, oxidative phosphorylation, and the role of 2-DG Ziqi Guo, Yaping Liu, Xin Li, Yuying Huang, Zuping Zhou & Cheng Yang Biology Direct volume 19, Article number: 73 (2024) Cite this article 1042 Accesses 2 Citation...
spectrum of energy metabolism, featured with elevated transcriptomes for key rate-limiting enzymes involved in oxidative phosphorylation, as well as the tricarboxylic acid (TCA) cycle and glycolysis. Moreover, these cKO microglia showed a panel of upregulated genes for phagocytosis and liver X receptor...
Assessment of oxidative phosphorylation and glycolysis in LSECs.AA heat map of the relative abundance (emPAI%) of selected proteins identified in LSECs related to the TCA cycle, ETC, FAO in mitochondria, MAS, glutamine metabolism (G), peroxisomes and FAO in peroxisomes (PX) and glycolysis. The...
we demonstrated that the aortic tissues of CKD mice had a preference for using oxidative phosphorylation (OXPHOS). Both high phosphate and human uremic serum-stimulated vascular smooth muscle cells (VSMCs) had enhanced mitochondrial respiration capacity, while the glycolysis level was not significantly...
To assess the functional activity of macrophages in terms of oxidative phosphorylation and glycolysis, the real-time mitochondrial oxygen consumption rates (OCRs) and the real-time extracellular acidification rates (ECARs) were measured using the XF96 Seahorse analyzer (Agilent Technologies, USA) accord...
Oxidative phosphorylation was evaluated by JC-1 staining and oxygen consumption rate (OCR) assay. Fatty acid oxidation assay was conducted to measure the status of RGCC-mediated fatty acid oxidation. NADPH and ROS levels were detected by well-established assays. The chemical sensitivity of cells ...
efferocytosis, oligomycin was added into the culture medium. The results showed that the ratio of macrophage with phagocyted neutrophil was significantly decreased after inhibition of complex V (Fig.4A, B). Therefore, SHH might enhance macrophage efferocytosis by promoting oxidative phosphorylation of ...
Oxidative phosphorylation was evaluated by JC-1 staining and oxygen consumption rate (OCR) assay. Fatty acid oxidation assay was conducted to measure the status of RGCC-mediated fatty acid oxidation. NADPH and ROS levels were detected by well-established assays. The chemical sensitivity of cells ...
To thoroughly examine the metabolic properties of the two trophoblast cell types, we compared their capacity for oxidative phosphorylation and glycolysis with different fuel substrates, and measured their intracellular ATP levels. We also tested the hypothesis that activity-sensitive mitochondrial dyes could...